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Plasmid Files

pLVX-Hom-Mem1

Lentiviral vector encoding FKBP homodimerizer domains, for creating membrane-associated fusion proteins that can be dimerized with a drug. 

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pLVX-Hom-Mem1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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XcmI (8698) SgrDI (8473) SspI (8356) PvuI (7922) FspI (7774) AhdI (7552) DrdI (6770) BmtI (6278) NheI (6274) PstI (5368) MluI (4750) SexAI * (4717) MscI * (4525) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) NdeI (2374) EcoRI (2803) SpeI (2815) AfeI (2860) XbaI (2863) BlpI (3083) NotI (3519) BamHI (3526) PspOMI (3657) ApaI (3661) AvrII (3695) BmgBI (4087) PflFI - Tth111I (4192) BsiWI (4206) RsrII (4266) BstEII (4284) pLVX-Hom-Mem1 8841 bp
XcmI  (8698)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8473)
1 site
C G T C G A C G G C A G C T G C
SspI  (8356)
1 site
A A T A T T T T A T A A
PvuI  (7922)
1 site
C G A T C G G C T A G C
FspI  (7774)
1 site
T G C G C A A C G C G T
AhdI  (7552)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6770)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (6278)
1 site
G C T A G C C G A T C G
NheI  (6274)
1 site
G C T A G C C G A T C G
PstI  (5368)
1 site
C T G C A G G A C G T C
MluI  (4750)
1 site
A C G C G T T G C G C A
SexAI  (4717)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MscI  (4525)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
NdeI  (2374)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
EcoRI  (2803)
1 site
G A A T T C C T T A A G
SpeI  (2815)
1 site
A C T A G T T G A T C A
AfeI  (2860)
1 site
A G C G C T T C G C G A
XbaI  (2863)
1 site
T C T A G A A G A T C T
BlpI  (3083)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (3519)
1 site
G C G G C C G C C</