Resources
Plasmid Files

pLVX-Tet3G

Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLVX-Tet3G Sequence and MappLVX-Tet3G.dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 SgrDI (8683) SspI (8566) PvuI (8132) AhdI (7762) BmtI (6485) NheI (6481) Bsu36I (5691) SacII (5473) PfoI (5243) MluI (4957) RsrII (4806) SphI (4695) PflFI - Tth111I (4408) FseI (1150) EcoNI (1170) BbvCI - Bpu10I (1424) BstAPI (1453) AleI (1577) KflI - PpuMI (1934) cPPT/CTS BspDI - ClaI (2180) SnaBI (2480) BsmBI (2729) EcoRI (2803) AbsI - PaeR7I - PspXI - XhoI (2809) XbaI (2824) TspMI - XmaI (3559) SmaI (3561) BamHI (3568) AvrII (3727) AarI (3915) BmgBI (4118) BstXI (4161) MscI (4372) pLVX-Tet3G 9051 bp
SgrDI  (8683)
1 site
C G T C G A C G G C A G C T G C
SspI  (8566)
1 site
A A T A T T T T A T A A
PvuI  (8132)
1 site
C G A T C G G C T A G C
AhdI  (7762)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmtI  (6485)
1 site
G C T A G C C G A T C G
NheI  (6481)
1 site
G C T A G C C G A T C G
Bsu36I  (5691)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SacII  (5473)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PfoI  (5243)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
MluI  (4957)
1 site
A C G C G T T G C G C A
RsrII  (4806)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
SphI  (4695)
1 site
G C A T G C C G T A C G
PflFI  (4408)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4408)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstAPI  (1453)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
SnaBI  (2480)
1 site
T A C G T A A T G C A T
BsmBI  (2729)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
EcoRI  (2803)
1 site
G A A T T C C T T A A G
AbsI  (2809)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (2809)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2809)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2809)
1 site
C T C G A G G A G C T C
XbaI  (2824)
1 site
T C T A G A A G A T C T
TspMI  (3559)
1 site
C C C G G G G G G C C C
XmaI  (3559)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (3561)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (3568)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
AvrII  (3727)
1 site
C C T A G G G G A T C C
AarI  (3915)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BmgBI  (4118)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BstXI  (4161)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
MscI  (4372)
1 site
T G G C C A A C C G G T
AmpR
7689 .. 8549  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   7689 .. 8480  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7689 .. 8549  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   8481 .. 8549  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7689 .. 8549  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
NeoR/KanR
4162 .. 4956  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
4162 .. 4956  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
Tet-On 3G
2821 .. 3567  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to
promoters containing the tet operator in the
presence of doxycycline
Tet-On 3G
2821 .. 3567  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to
promoters containing the tet operator in the
presence of doxycycline
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
3' LTR
5766 .. 6399  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5766 .. 6399  =  634 bp
3' long terminal repeat (LTR) from HIV-1
WPRE
4970 .. 5558  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
WPRE
4970 .. 5558  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
ori
6930 .. 7518  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6930 .. 7518  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
IRES
3576 .. 4148  =  573 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
IRES
3576 .. 4148  =  573 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
8702 .. 8836  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8702 .. 8836  =  135 bp
SV40 polyadenylation signal
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
AmpR promoter
8550 .. 8654  =  105 bp
AmpR promoter
8550 .. 8654  =  105 bp
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter