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pLenti-C-Myc-DDK-IRES-BSD

Third-generation lentiviral PrecisionShuttle™ vector with a blasticidin resistance marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags.

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pLenti-C-Myc-DDK-IRES-BSD.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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BsmBI (6823) PasI (6816) ScaI (6634) Bsu36I (5304) SacII (5085) BsrGI (4464) BsaBI * (4363) BclI * (4058) BmgBI (4033) AarI - BfuAI - BspMI (3829) SgrDI (1) KasI (1017) NarI (1018) SfoI (1019) PluTI (1021) BspQI - SapI (1482) FseI (1530) EcoNI (1550) BbvCI (1804) AleI (1957) PstI (2417) XbaI (2613) EcoRI (3279) BamHI (3292) AsiSI - SgfI (3324) MreI - SgrAI (3326) AscI (3330) NheI (3355) BmtI (3359) RsrII (3361) MluI (3367) BsiWI (3370) PaeR7I - PspXI - XhoI (3382) PmeI (3460) PmlI (3806) pLenti-C-Myc-DDK-IRES-BSD 7398 bp
BsmBI  (6823)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (6816)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
ScaI  (6634)
1 site
A G T A C T T C A T G A
Bsu36I  (5304)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SacII  (5085)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BsrGI  (4464)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BsaBI  (4363)
1 site
G A T N N N N A T C C T A N N