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Plasmid Files

pLenti-N-tYFP

Third-generation lentiviral PrecisionShuttle™ vector for expression of an ORF tagged at the N-terminus with TurboYFP.

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pLenti-N-tYFP Sequence and MappLenti-N-tYFP.dna
Map and Sequence File   
Sequence Author:  OriGene
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 AclI (6640) BsmBI (6501) PasI (6494) ScaI (6312) DrdI (5666) Bsu36I (4982) PfoI (4533) PmeI (4123) PaeR7I - PspXI - XhoI (4114) NotI (4106) MluI (4096) RsrII (4090) AscI (4059) MreI (4055) AsiSI - SgfI (4053) BlpI (3938) SphI (3577) SgrDI (1) CMV enhancer EcoNI (1550) BbvCI (1804) PstI (2417) PspOMI (2605) ApaI (2609) XbaI (2613) CMV enhancer EcoRI (3279) BamHI (3292) pLenti-N-tYFP 7076 bp
AclI  (6640)
1 site
A A C G T T T T G C A A
BsmBI  (6501)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (6494)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
ScaI  (6312)
1 site
A G T A C T T C A T G A
DrdI  (5666)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Bsu36I  (4982)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PfoI  (4533)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PmeI  (4123)
1 site
G T T T A A A C C A A A T T T G
PaeR7I  (4114)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (4114)
1 site
V C T C G A G B B G A G C T C V
XhoI  (4114)
1 site
C T C G A G G A G C T C
NotI  (4106)
1 site
G C G G C C G C C G C C G G C G
MluI  (4096)
1 site
A C G C G T T G C G C A
RsrII  (4090)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AscI  (4059)
1 site
G G C G C G C C C C G C G C G G
MreI  (4055)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (4053)
1 site
G C G A T C G C C G C T A G C G
SgfI  (4053)
1 site
G C G A T C G C C G C T A G C G
BlpI  (3938)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SphI  (3577)
1 site
G C A T G C C G T A C G
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
PstI  (2417)
1 site
C T G C A G G A C G T C
PspOMI  (2605)
1 site
G G G C C C C C C G G G
ApaI  (2609)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
XbaI  (2613)
1 site
T C T A G A A G A T C T
EcoRI  (3279)
1 site
G A A T T C C T T A A G
BamHI  (3292)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
TurboYFP
3320 .. 4048  =  729 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
TurboYFP
3320 .. 4048  =  729 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
CmR
6284 .. 6943  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
6284 .. 6943  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
WPRE
4260 .. 4848  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
WPRE
4260 .. 4848  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
ori
5619 .. 6207  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5619 .. 6207  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2643 .. 3022  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2643 .. 3022  =  380 bp
human cytomegalovirus immediate early enhancer
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
CMV promoter
3023 .. 3226  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
3023 .. 3226  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early
promoter
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
3' LTR (truncated)
5377 .. 5557  =  181 bp
truncated 3' long terminal repeat (LTR) from HIV-1
3' LTR (truncated)
5377 .. 5557  =  181 bp
truncated 3' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
AmpR promoter
6944 .. 7048  =  105 bp
AmpR promoter
6944 .. 7048  =  105 bp
MCS
4049 .. 4127  =  79 bp
multiple cloning site
MCS
4049 .. 4127  =  79 bp
multiple cloning site
loxP
4171 .. 4204  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
4171 .. 4204  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
T7 promoter
3252 .. 3270  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
3252 .. 3270  =  19 bp
promoter for bacteriophage T7 RNA polymerase
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