Resources
Plasmid Files

pLenti6.2/N-Lumio™/V5-DEST

Lentiviral Gateway® destination vector for N-terminal tagging of a protein with the Lumio™ tetracysteine tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLenti6.2 N-Lumio V5-DEST Sequence and MappLenti6.2 N-Lumio V5-DEST.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 PciI (8546) AhdI (7658) DraIII (6518) NaeI (6412) NgoMIV (6410) AvrII (6077) SfiI (6030) SV40 poly(A) signal PmlI (4923) PGK promoter SpeI (4658) EcoNI (942) MfeI (961) BstAPI (1225) AleI (1349) KflI (1706) BspDI - ClaI (1795) NdeI (2055) SnaBI (2161) CMV promoter tetracysteine tag lac UV5 promoter EcoRI (3028) PasI - PflMI * (3260) BstZ17I (3563) TspMI - XmaI (3897) SmaI - SrfI (3899) BfuAI - BspMI (4143) PstI (4154) EcoRV (4290) PaeR7I - PspXI - XhoI (4311) PspOMI (4323) ApaI (4327) SacII (4330) BstBI (4334) MluI (4378) pLenti6.2/N-Lumio™/V5-DEST 8956 bp
PciI  (8546)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (7658)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (6518)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (6412)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (6410)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
AvrII  (6077)
1 site
C C T A G G G G A T C C
SfiI  (6030)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PmlI  (4923)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
SpeI  (4658)
1 site
A C T A G T T G A T C A
EcoNI  (942)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (961)
1 site
C A A T T G G T T A A C
BstAPI  (1225)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AleI  (1349)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1706)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (1795)
1 site
A T C G A T T A G C T A
ClaI  (1795)
1 site
A T C G A T T A G C T A
NdeI  (2055)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (2161)
1 site
T A C G T A A T G C A T
EcoRI  (3028)
1 site
G A A T T C C T T A A G
PasI  (3260)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (3260)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BstZ17I  (3563)
1 site
G T A T A C C A T A T G
TspMI  (3897)
1 site
C C C G G G G G G C C C
XmaI  (3897)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (3899)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SrfI  (3899)
1 site
G C C C G G G C C G G G C C C G
BfuAI  (4143)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4143)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (4154)
1 site
C T G C A G G A C G T C
EcoRV  (4290)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
PaeR7I  (4311)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (4311)
1 site
V C T C G A G B B G A G C T C V
XhoI  (4311)
1 site
C T C G A G G A G C T C
PspOMI  (4323)
1 site
G G G C C C C C C G G G
ApaI  (4327)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SacII  (4330)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BstBI  (4334)
1 site
T T C G A A A A G C T T
MluI  (4378)
1 site
A C G C G T T G C G C A
AmpR
6871 .. 7731  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6871 .. 6939  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6871 .. 7731  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6940 .. 7731  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6871 .. 7731  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
2815 .. 3474  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2815 .. 3474  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
7902 .. 8490  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
7902 .. 8490  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
PGK promoter
4413 .. 4912  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
4413 .. 4912  =  500 bp
mouse phosphoglycerate kinase 1 promoter
f1 ori
6285 .. 6740  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
6285 .. 6740  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
BSD
4992 .. 5390  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
4992 .. 5390  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
ccdB
3816 .. 4121  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
3816 .. 4121  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
CMV enhancer
1882 .. 2185  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1882 .. 2185  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1075 .. 1308  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1075 .. 1308  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
3' LTR (ΔU3)
5477 .. 5710  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from
HIV-1
3' LTR (ΔU3)
5477 .. 5710  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from
HIV-1
RSV promoter
1 .. 229  =  229 bp
Rous sarcoma virus enhancer/promoter
RSV promoter
1 .. 229  =  229 bp
Rous sarcoma virus enhancer/promoter
CMV promoter
2186 .. 2389  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
2186 .. 2389  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
5' LTR (truncated)
230 .. 410  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
230 .. 410  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
457 .. 582  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
457 .. 582  =  126 bp
packaging signal of human immunodeficiency virus
type 1
attR1
2582 .. 2706  =  125 bp
recombination site for the Gateway® LR reaction
attR1
2582 .. 2706  =  125 bp
recombination site for the Gateway® LR reaction
attR2
4162 .. 4286  =  125 bp
recombination site for the Gateway® LR reaction
attR2
4162 .. 4286  =  125 bp
recombination site for the Gateway® LR reaction
SV40 poly(A) signal
5782 .. 5903  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5782 .. 5903  =  122 bp
SV40 polyadenylation signal
AmpR promoter
6766 .. 6870  =  105 bp
AmpR promoter
6766 .. 6870  =  105 bp
EM7 promoter
4926 .. 4973  =  48 bp
synthetic bacterial promoter
EM7 promoter
4926 .. 4973  =  48 bp
synthetic bacterial promoter
V5 tag
2528 .. 2569  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
2528 .. 2569  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
lac UV5 promoter
2731 .. 2761  =  31 bp
   Segment 1:  -35  
   2731 .. 2736  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2731 .. 2761  =  31 bp
   Segment 2:  
   2737 .. 2754  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2731 .. 2761  =  31 bp
   Segment 3:  -10  
   2755 .. 2761  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2731 .. 2761  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
tetracysteine tag
2495 .. 2512  =  18 bp
6 amino acids  =  584.8 Da
Product: tetracysteine peptide that binds biarsenical
labeling reagents
tetracysteine tag
2495 .. 2512  =  18 bp
6 amino acids  =  584.8 Da
Product: tetracysteine peptide that binds biarsenical
labeling reagents
ATG
2483 .. 2485  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2483 .. 2485  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter