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pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro

Lentiviral vector for coexpressing Cas9 from the ubiquitin C promoter together with a single guide RNA (sgRNA), for permanently knocking out a target gene.

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pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Cellecta
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SspI (10,966) PciI (9269) SfiI (8847) AanI (8618) AanI (8562) KpnI (8227) Acc65I (8223) HincII (7628) SalI (7626) SexAI * (7587) BstEII (7154) RsrII (7136) BsiWI (7076) PflFI - Tth111I (7062) BamHI (6954) NotI (6946) FseI (6925) BsaBI * (6291) MluI (1) NruI * (612) MfeI (964) BbvCI (1199) AleI (1352) BspDI - ClaI (1798) BbsI - BpiI (2064) BbsI - BpiI (2090) PaeR7I - PspXI - XhoI (2113) EcoRI (2280) AgeI (2289) BstBI (2298) AfeI (2358) PspOMI (4148) ApaI (4152) EcoRV (4646) PmlI (5582)