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pRetroQ-DsRed-Monomer-N1

Self-inactivating retroviral vector for expressing a protein fused to the N-terminus of DsRed-Monomer.

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pRetroQ-DsRed-Monomer-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SspI (7226) XmnI (7021) ScaI (6902) AflIII - PciI (5529) BspQI - SapI (5413) AvrII (5282) SfiI (5235) EcoRV (4158) RsrII (3602) AscI (542) PshAI (676) NheI (2172) BmtI (2176) PaeR7I - XhoI (2194) BstBI (2208) EcoRI (2210) SalI (2220) AccI (2221) PspOMI (2234) ApaI (2238) BamHI (2241) FspAI (2308) SbfI (2600) NgoMIV (2680) NaeI (2682) PflMI (2841) AleI (2887) NotI (2940) XbaI (2950) BlpI (3239) BsmI (3397) BsiWI (3542) pRetroQ-DsRed-Monomer-N1 7571 bp
SspI  (7226)
1 site
A A T A T T T T A T A A
XmnI  (7021)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6902)
1 site
A G T A C T T C A T G A
AflIII  (5529)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5529)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5413)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5413)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (5282)
1 site
C C T A G G G G A T C C
SfiI  (5235)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (4158)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
RsrII  (3602)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AscI  (542)
1 site
G G C G C G C C C C G C G C G G
PshAI  (676)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NheI  (2172)
1 site
G C T A G C C G A T C G