Plasmid Files

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SspI  (7791) 1 site

ScaI  (7467) 1 site

PvuI  (7357) 1 site

AlwNI  (6510) 1 site

Sticky ends from different AlwNI sites may not be compatible.

SfiI  (5800) 1 site

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

BmtI  (4876) 1 site

NheI  (4872) 1 site

EcoRV  (4730) 1 site

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.

BsgI  (4639) 1 site

Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

PaeR7I  (4589) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

PspXI  (4589) 1 site

XhoI  (4589) 1 site

RsrII  (4275) 1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible. For full activity, add fresh DTT.

NaeI  (4261) 1 site

Efficient cleavage requires at least two copies of the NaeI recognition sequence.

AscI  (542) 1 site

PshAI  (676) 1 site

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

BstEII  (1252) 1 site

Sticky ends from different BstEII sites may not be compatible. BstEII is typically used at 60°C, but is 50% active at 37°C.

Bpu10I  (1455) 1 site

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site. Sticky ends from different Bpu10I sites may not be compatible.

BglII  (1576) 1 site

SbfI  (2230) 1 site

NotI  (2243) 1 site

AgeI  (2250) 1 site

AgeI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

BsiWI  (2262) 1 site

BsiWI is typically used at 55°C, but is 50% active at 37°C.

PacI  (2273) 1 site

BamHI  (2278) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

XcmI  (2295) 1 site

The 1-base overhangs produced by XcmI may be hard to ligate. Sticky ends from different XcmI sites may not be compatible.

NruI  (2570) 1 site

MfeI  (2807) 1 site

EcoRI  (3037) 1 site

AarI  (3384) 1 site

Efficient cleavage requires at least two copies of the AarI recognition sequence. Sticky ends from different AarI sites may not be compatible. After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.

PflMI  (3498) 1 site

Sticky ends from different PflMI sites may not be compatible.

BmgBI  (3587) 1 site

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.

BstXI  (3630) 1 site

Sticky ends from different BstXI sites may not be compatible.

NgoMIV  (4259) 1 site

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.

AmpR 6914 .. 7774  =  861 bp 286 amino acids  =  31.5 kDa    Segment 2:      6914 .. 7705  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 6914 .. 7774  =  861 bp 286 amino acids  =  31.5 kDa    Segment 1:  signal sequence      7706 .. 7774  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 6914 .. 7774  =  861 bp 286 amino acids  =  31.5 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

NeoR/KanR 3631 .. 4425  =  795 bp 264 amino acids  =  29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin)

NeoR/KanR 3631 .. 4425  =  795 bp 264 amino acids  =  29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin)

Tet-On® 3G 2290 .. 3036  =  747 bp 248 amino acids  =  27.7 kDa Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline

Tet-On® 3G 2290 .. 3036  =  747 bp 248 amino acids  =  27.7 kDa Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline

ori 6155 .. 6743  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 6155 .. 6743  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

IRES 3045 .. 3617  =  573 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)

IRES 3045 .. 3617  =  573 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)

3' LTR (ΔU3) 4842 .. 5267  =  426 bp self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus

3' LTR (ΔU3) 4842 .. 5267  =  426 bp self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus

gag (truncated) 1151 .. 1567  =  417 bp truncated MMLV gag gene lacking the start codon

gag (truncated) 1151 .. 1567  =  417 bp truncated MMLV gag gene lacking the start codon

CMV enhancer 8065 .. 308  =  380 bp human cytomegalovirus immediate early enhancer

CMV enhancer 8065 .. 308  =  380 bp human cytomegalovirus immediate early enhancer

SV40 promoter 5533 .. 5862  =  330 bp SV40 enhancer and early promoter

SV40 promoter 5533 .. 5862  =  330 bp SV40 enhancer and early promoter

CMV enhancer 1604 .. 1907  =  304 bp human cytomegalovirus immediate early enhancer

CMV enhancer 1604 .. 1907  =  304 bp human cytomegalovirus immediate early enhancer

CMV promoter 309 .. 512  =  204 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 309 .. 512  =  204 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 1908 .. 2111  =  204 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 1908 .. 2111  =  204 bp human cytomegalovirus (CMV) immediate early promoter

MMLV Ψ 751 .. 950  =  200 bp packaging signal of Moloney murine leukemia virus (MMLV)

MMLV Ψ 751 .. 950  =  200 bp packaging signal of Moloney murine leukemia virus (MMLV)

5' LTR (truncated) 513 .. 688  =  176 bp truncated long terminal repeat from Moloney murine sarcoma virus

5' LTR (truncated) 513 .. 688  =  176 bp truncated long terminal repeat from Moloney murine sarcoma virus

AmpR promoter 7775 .. 7879  =  105 bp

AmpR promoter 7775 .. 7879  =  105 bp

SV40 ori 5713 .. 5848  =  136 bp SV40 origin of replication

SV40 ori 5713 .. 5848  =  136 bp SV40 origin of replication

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