Sticky ends from different AlwNI sites may not be compatible.
SfiI (5800) 1 site
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BmtI (4876) 1 site
GCTAGCCGATCG
NheI (4872) 1 site
GCTAGCCGATCG
EcoRV (4730) 1 site
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BsgI (4639) 1 site
GTGCAG(N)14NNCACGTC(N)14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
PaeR7I (4589) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI (4589) 1 site
VCTCGAGBBGAGCTCV
XhoI (4589) 1 site
CTCGAGGAGCTC
RsrII (4275) 1 site
CGGWCCGGCCWGGC
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
NaeI (4261) 1 site
GCCGGCCGGCCG
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
AscI (542) 1 site
GGCGCGCCCCGCGCGG
PshAI (676) 1 site
GACNNNNGTCCTGNNNNCAG
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BstEII (1252) 1 site
GGTNACCCCANTGG
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C.
Bpu10I (1455) 1 site
CCTNAGCGGANTCG
Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.