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pTRE3G-Hom1

Mammalian vector encoding a doxycycline-inducible FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug. 

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pTRE3G-Hom1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3770) AatII (3699) ZraI (3697) XmnI (3376) ScaI (3257) TatI (3255) FspI (2999) NmeAIII (2925) BglI (2897) BsrFI (2857) BsaI (2838) AhdI (2777) AlwNI (2300) DrdI (1992) PaeR7I - PspXI - XhoI (1) HindIII (253) KasI (274) NarI (275) SfoI (276) PluTI (278) Eco53kI (292) SacI (294) SalI (383) AccI (384) PspOMI (393) ApaI (397) BglII (399) BspDI * - ClaI * (406) EagI (411) EcoRV (419) AarI (431) BsmBI (457) TspMI - XmaI (550) SmaI (552) MluI (756) NdeI (763) NheI (768) BmtI (772) PstI - SbfI (778) BamHI (780) BtgZI (828) PflMI (1002) StyI (1150) BsaBI * (1465) MfeI (1553) HpaI (1566) BspQI - SapI (1768) PciI (1884) pTRE3G-Hom1 3775 bp
EcoRI  (3770)
1 site
G A A T T C C T T A A G
AatII  (3699)
1 site
G A C G T C C T G C A G
ZraI  (3697)
1 site
G A C G T C C T G C A G
XmnI  (3376)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3257)
1 site
A G T A C T T C A T G A
TatI  (3255)
1 site
W G T A C W W C A T G W
FspI  (2999)
1 site
T G C G C A A C G C G T
NmeAIII  (2925)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2897)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2857)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2838)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2777)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2300)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
DrdI  (1992)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PaeR7I  (1)
1 site
C T C G A G G A G C T C