pZIP-mCMV-RFP-Puro

Lentiviral vector with a mouse CMV promoter-driven cassette encoding TurboRFP plus a short hairpin RNA (shRNA) with an "UltramiR" microRNA scaffold.

Sequence Author: transOMIC

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pZIP-mCMV-RFP-Puro 8308 bp
AmpR
7312 .. 8172  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7312 .. 8103  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7312 .. 8172  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8104 .. 8172  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7312 .. 8172  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TurboRFP
2768 .. 3463  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
TurboRFP
2768 .. 3463  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
PuroR
4052 .. 4654  =  603 bp
200 amino acids  =  21.6 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
4052 .. 4654  =  603 bp
200 amino acids  =  21.6 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
5013 .. 5601  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
5013 .. 5601  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
6556 .. 7141  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6556 .. 7141  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
3477 .. 4049  =  573 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
3477 .. 4049  =  573 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
mCMV promoter
2219 .. 2755  =  537 bp
mouse cytomegalovirus (CMV) immediate early promoter
mCMV promoter
2219 .. 2755  =  537 bp
mouse cytomegalovirus (CMV) immediate early promoter
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
6138 .. 6467  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
6138 .. 6467  =  330 bp
SV40 enhancer and early promoter
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
RRE
1337 .. 1570  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1337 .. 1570  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
3' LTR (ΔU3)
5809 .. 6042  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
5809 .. 6042  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' miR-30a
4866 .. 4992  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
3' miR-30a
4866 .. 4992  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
5' miR-30a
4675 .. 4796  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
4675 .. 4796  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
cPPT/CTS
2062 .. 2177  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2062 .. 2177  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
8173 .. 8277  =  105 bp
AmpR promoter
8173 .. 8277  =  105 bp
shRNA
4797 .. 4865  =  69 bp
3 segments
   Segment 1:  
   4797 .. 4823  =  27 bp
short hairpin RNA
shRNA
4797 .. 4865  =  69 bp
3 segments
   Segment 2:  miR-30a loop  
   4824 .. 4838  =  15 bp
short hairpin RNA
shRNA
4797 .. 4865  =  69 bp
3 segments
   Segment 3:  
   4839 .. 4865  =  27 bp
short hairpin RNA
shRNA
4797 .. 4865  =  69 bp
3 segments
short hairpin RNA
SV40 ori
6318 .. 6453  =  136 bp
SV40 origin of replication
SV40 ori
6318 .. 6453  =  136 bp
SV40 origin of replication
ORF:  5116 .. 5412  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  2768 .. 3463  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  4052 .. 4654  =  603 bp
ORF:  200 amino acids  =  21.6 kDa
ORF:  7442 .. 7708  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1215 .. 2066  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  3960 .. 4349  =  390 bp
ORF:  129 amino acids  =  13.8 kDa
ORF:  5103 .. 5630  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  1049 .. 1510  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  3965 .. 4717  =  753 bp
ORF:  250 amino acids  =  26.1 kDa
ORF:  2869 .. 3114  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  7312 .. 8172  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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