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Plasmid Files

kanMX6

kanMX selector module conferring kanamycin resistance, for gene disruption in yeast, kanMX6 version.

 
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 1250 1000 750 500 250 kanMX TEF promoter KanR TEF terminator End (1430) EcoP15I (1366) ScaI (1198) TatI (1196) PflMI (1072) TaqII (1063) BstYI (1058) AseI (1008) BsaWI (944) HindIII (927) BsmBI (825) AsiSI - BsiEI - PvuI (809) BsrFI (763) SspI (734) EcoNI (721) TsoI (650) EarI (622) BspDI - ClaI (500) NruI (466) BanII - Bsp1286I (464) BtgI - NcoI - StyI (382) BciVI (340) DraI (333) AflIII - MluI (242) BfuAI - BspMI (220) PstI (209) BseRI (189) BmgBI (78) BstXI (42) BstEII (25) Start (0) kanMX6 1430 bp
End  (1430)
0 sites
EcoP15I  (1366)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
ScaI  (1198)
1 site
A G T A C T T C A T G A
TatI  (1196)
1 site
W G T A C W W C A T G W
PflMI  (1072)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
TaqII  (1063)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BstYI  (1058)
1 site
R G A T C Y Y C T A G R
AseI  (1008)
1 site
A T T A A T T A A T T A
BsaWI  (944)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
HindIII  (927)
1 site
A A G C T T T T C G A A
BsmBI  (825)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AsiSI  (809)
1 site
G C G A T C G C C G C T A G C G
BsiEI  (809)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
PvuI  (809)
1 site
C G A T C G G C T A G C
BsrFI  (763)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SspI  (734)
1 site
A A T A T T T T A T A A
EcoNI  (721)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
TsoI  (650)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (622)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BspDI  (500)
1 site
A T C G A T T A G C T A
ClaI  (500)
1 site
A T C G A T T A G C T A
NruI  (466)
1 site
T C G C G A A G C G C T
BanII  (464)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (464)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BtgI  (382)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (382)
1 site
C C A T G G G G T A C C
StyI  (382)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BciVI  (340)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
DraI  (333)
1 site
T T T A A A A A A T T T
AflIII  (242)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (242)
1 site
A C G C G T T G C G C A
BfuAI  (220)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (220)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (209)
1 site
C T G C A G G A C G T C
BseRI  (189)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BmgBI  (78)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BstXI  (42)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BstEII  (25)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
Start  (0)
0 sites
kanMX
40 .. 1396  =  1357 bp
yeast selectable marker conferring kanamycin
resistance (Wach, 1996)
kanMX
40 .. 1396  =  1357 bp
yeast selectable marker conferring kanamycin
resistance (Wach, 1996)
TEF promoter
40 .. 383  =  344 bp
Ashbya gossypii TEF promoter
TEF promoter
40 .. 383  =  344 bp
Ashbya gossypii TEF promoter
KanR
384 .. 1193  =  810 bp
269 amino acids  =  30.7 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418
(Geneticin®) in eukaryotes
KanR
384 .. 1193  =  810 bp
269 amino acids  =  30.7 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418
(Geneticin®) in eukaryotes
TEF terminator
1199 .. 1396  =  198 bp
Ashbya gossypii TEF terminator
TEF terminator
1199 .. 1396  =  198 bp
Ashbya gossypii TEF terminator
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