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Plasmid Files

pFA6a-His3MX6-PGAL1-GFP

Plasmid with a HIS3MX6 marker for swapping in the GAL1 promoter and adding a GFP tag.

 
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pFA6a-His3MX6-PGAL1-GFP.dna
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HindIII (19) PfoI (5027) EcoO109I (4970) AatII (4916) ZraI (4914) SspI (4798) XmnI (4593) ScaI (4474) PvuI (4364) BmrI (4034) BanI (3942) BspQI - SapI (2985) SacII (2870) SfiI (2863) EcoRV (2840) F4 (2812 .. 2831) EcoRI (2826) SacI (2824) Eco53kI (2822) PmeI (2815) BsiWI (25) SalI (37) AscI (251) R5 (261 .. 280) BstBI (350) MfeI (410) PmlI (649) BsrGI (694) SnaBI (780) MscI (803) PacI (977) BstAPI (1128) AgeI (1373) BglII (1536) BstEII (1566) BmgBI (1619) Bpu10I (1628) MluI (1783) SphI (2051) BclI * (2182) BsaBI (2184) NgoMIV (2252) NaeI (2254) XbaI (2303) AfeI (2491) pFA6a-His3MX6-PGAL1-GFP 5248 bp
HindIII  (19)
1 site
A A G C T T T T C G A A
PfoI  (5027)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
EcoO109I  (4970)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4916)
1 site
G A C G T C C T G C A G
ZraI  (4914)
1 site
G A C G T C C T G C A G
SspI  (4798)
1 site
A A T A T T T T A T A A
XmnI  (4593)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4474)
1 site
A G T A C T T C A T G A
PvuI  (4364)
1 site
C G A T C G G C T A G C
BmrI  (4034)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BanI  (3942)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BspQI  (2985)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2985)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SacII  (2870)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
SfiI  (2863)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (2840)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EcoRI  (2826)
1 site
G A A T T C C T T A A