Sticky ends from different EcoO109I sites may not be compatible.
SspI (3825) 1 site
AATATTTTATAA
ScaI (3501) 1 site
AGTACTTCATGA
PvuI (3391) 1 site
CGATCGGCTAGC
FspI (3243) 1 site
TGCGCAACGCGT
BpmI (3091) 1 site
CTGGAG(N)14NNGACCTC(N)14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
BanI (2969) 1 site
GGYRCCCCRYGG
Sticky ends from different BanI sites may not be compatible.
AlwNI (2544) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
AflIII (2128) 1 site
ACRYGTTGYRCA
Sticky ends from different AflIII sites may not be compatible.
PciI (2128) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
BsiWI (25) 1 site
CGTACGGCATGC
BsiWI is typically used at 55°C, but is 50% active at 37°C.
PstI (35) 1 site
CTGCAGGACGTC
SalI (37) 1 site
GTCGACCAGCTG
PsiI (194) 1 site
TTATAAAATATT
AscI (251) 1 site
GGCGCGCCCCGCGCGG
BssHII (251) 1 site
GCGCGCCGCGCG
BssHII is typically used at 50°C, but is 75% active at 37°C.
BbvCI (257) 1 site
CCTCAGCGGAGTCG
Bpu10I (257) 1 site
CCTNAGCGGANTCG
Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.
BamHI (297) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PacI (371) 1 site
TTAATTAAAATTAATT
BtgZI (541) 1 site
GCGATG(N)10CGCTAC(N)10(N)4
Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C.
AgeI (767) 1 site
ACCGGTTGGCCA
BseRI (805) 1 site
GAGGAG(N)8NNCTCCTC(N)8
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA.
BglII (930) 1 site
AGATCTTCTAGA
BsrGI (935) 1 site
TGTACAACATGT
BsrGI is typically used at 37°C, but is even more active at 60°C.
BspEI (948) 1 site
TCCGGAAGGCCT
* Blocked by Dam methylation.
PmlI (1043) 1 site
CACGTGGTGCAC
PmlI gradually loses activity when stored at -20°C.
XbaI (1167) 1 site
TCTAGAAGATCT
MfeI (1223) 1 site
CAATTGGTTAAC
Bsu36I (1325) 1 site
CCTNAGGGGANTCC
Sticky ends from different Bsu36I sites may not be compatible.
BstXI (1381) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
BsgI (1496) 1 site
GTGCAG(N)14NNCACGTC(N)14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
AarI (1516) 1 site
CACCTGC(N)4GTGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PmeI (1842) 1 site
GTTTAAACCAAATTTG
Eco53kI (1849) 1 site
GAGCTCCTCGAG
SacI (1851) 1 site
GAGCTCCTCGAG
EcoRI (1853) 1 site
GAATTCCTTAAG
BspDI (1860) 1 site
ATCGATTAGCTA
ClaI (1860) 1 site
ATCGATTAGCTA
SpeI (1877) 1 site
ACTAGTTGATCA
SfiI (1890) 1 site
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BtgI (1894) 1 site
CCRYGGGGYRCC
Sticky ends from different BtgI sites may not be compatible.
SacII (1897) 1 site
CCGCGGGGCGCC
Efficient cleavage requires at least two copies of the SacII recognition sequence.