Resources
Plasmid Files

pGilda

Centromeric yeast two-hybrid vector for galactose-regulated expression of LexA fused to the N-terminus of a protein.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pGilda.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
Download Free Trial Get SnapGene Viewer


PspOMI (6552) PspFI (5849) BseYI (5845) AlwNI (5740) AhdI (5261) BsaI (5195) ScaI (4780) BsaHI (4721) PpuMI (4338) PmlI (4327) SwaI (4303) PfoI (3708) AfeI (3548) ApaI (6556) AgeI (293) BstAPI (545) HpaI (702) MluI (770) EcoRV (1057) SnaBI (1109) PmeI (1213) XcmI (1258) EcoRI (1298) TspMI - XmaI (1303) SmaI (1305) BamHI (1308) BtgI - NcoI - StyI (1319) EagI - NotI (1325) PaeR7I - PspXI - XhoI (1331) BfuAI - BspMI (1349) SphI (1554) Eco53kI (1684) SacI (1686) NgoMIV (2007) NaeI (2009) DraIII (2115) BssHII (2444) NsiI (2481) AleI (2697) NheI (2748) BmtI (2752) BsiWI (2840) BstXI (2871) MscI (3049) BsaBI * (3199) pGilda 6570 bp
PspOMI  (6552)
1 site
G G G C C C C C C G G G
PspFI  (5849)
1 site
C C C A G C G G G T C G
BseYI  (5845)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (5740)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (5261)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (5195)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ScaI  (4780)
1 site
A G T A C T T C A T G A
BsaHI  (4721)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
PpuMI  (4338)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
PmlI  (4327)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
SwaI  (4303)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PfoI  (3708)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
AfeI  (3548)
1 site
A G C G C T T C G C G A
ApaI  (6556)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AgeI  (293)
1 site
A C C G G T T G G C C A
BstAPI  (545)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
HpaI  (702)
1 site
G T T A A C C A A T T G
MluI  (770)
1 site
A C G C G T T G C G C A
EcoRV  (1057)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
SnaBI  (1109)
1 site
T A C G T A A T G C A T
PmeI  (1213)
1 site
G T T T A A A C C A A A T T T G
XcmI  (1258)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoRI  (1298)
1 site
G A A T T C C T T A A G
TspMI  (1303)
1 site
C C C G G G G G G C C C
XmaI  (1303)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1305)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1308)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BtgI  (1319)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1319)
1 site
C C A T G G G G T A C C
StyI  (1319)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EagI  (1325)
1 site
C G G C C G G C C G G C
NotI  (1325)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1331)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1331)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1331)
1 site
C T C G A G G A G C T C
BfuAI  (1349)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1349)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (1554)
1 site
G C A T G C C G T A C G
Eco53kI  (1684)
1 site
G A G C T C C T C G A G
SacI  (1686)
1 site
G A G C T C C T C G A G
NgoMIV  (2007)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (2009)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
DraIII  (2115)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BssHII  (2444)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NsiI  (2481)
1 site
A T G C A T T A C G T A