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Plasmid Files

pRS305

Yeast integrative vector with a LEU2 marker and an MCS derived from pBLUESCRIPT.

 
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pRS305.dna
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AatII (5439) ZraI (5437) ScaI (4997) BsaI (4578) AhdI (4517) AlwNI (4040) BspQI - SapI (3508) ApaI (3224) PspOMI (3220) AbsI - PaeR7I - PspXI - XhoI (3211) SalI (3205) HindIII (3190) PstI (3176) SmaI (3168) TspMI - XmaI (3166) BamHI (3160) SpeI (3154) XbaI (3148) EagI - NotI (3141) SacII (3135) AleI (3134) SacI (3128) Eco53kI (3126) NaeI (2801) NgoMIV (2799) PfoI (46) PflFI - Tth111I (192) BsrGI (632) BfuAI - BspMI (759) XcmI (1201) BbsI (1301) AflII (1438) AgeI (1503) BstEII (1718) PpuMI (1875) HpaI (2174) KasI (2203) NarI (2204) SfoI (2205) PluTI (2207) BsaAI - DraIII (2698) BtgZI (2699) pRS305 5504 bp
AatII  (5439)
1 site
G A C G T C C T G C A G
ZraI  (5437)
1 site
G A C G T C C T G C A G
ScaI  (4997)
1 site
A G T A C T T C A T G A
BsaI  (4578)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4517)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (4040)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspQI  (3508)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3508)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
ApaI  (3224)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3220)
1 site
G G G C C C C C C G G G
AbsI  (3211)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (3211)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3211)
1 site
V C T C G A G B B G A G C T C V
XhoI  (3211)
1 site
C T C G A G G A G C T C
SalI  (3205)
1 site
G T C G A C C A G C T G
HindIII  (3190)
1 site
A A G C T T T T C G A A
PstI  (3176)
1 site
C T G C A G G A C G T C
SmaI  (3168)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (3166)
1 site
C C C G G G G G G C C C
XmaI  (3166)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BamHI  (3160)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SpeI  (3154)
1 site
A C T A G T T G A T C A
XbaI  (3148)
1 site
T C T A G A A G A T C T
EagI  (3141)
1 site
C G G C C G G C C G G C
NotI  (3141)
1 site
G C G G C C G C C G C C G G C G
SacII  (3135)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (3134)
1 site
C A C N N N N G T G G T G N N N N C A C
SacI  (3128)
1 site
G A G C T C C T C G A G
Eco53kI  (3126)
1 site
G A G C T C C T C G A G
NaeI  (2801)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2799)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PfoI  (46)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (192)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (192)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsrGI  (632)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BfuAI  (759)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (759)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
XcmI  (1201)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BbsI  (1301)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AflII  (1438)
1 site
C T T A A G G A A T T C
AgeI  (1503)
1 site
A C C G G T T G G C C A
BstEII  (1718)
1 site
G G T N A C C C C