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Plasmid Files

CMV promoter

Human cytomegalovirus (CMV) immediate early enhancer and promoter.

 
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 500 400 300 200 100 CMV enhancer CMV promoter End (508) EciI (436) BanI (405) MslI (305) BtgI - NcoI - StyI (300) BsaAI - SnaBI (280) BtgZI (274) BmrI (229) NdeI (174) TaqII (112) Start (0) CMV promoter 508 bp
End  (508)
0 sites
EciI  (436)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BanI  (405)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
MslI  (305)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BtgI  (300)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (300)
1 site
C C A T G G G G T A C C
StyI  (300)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsaAI  (280)
1 site
Y A C G T R R T G C A Y
SnaBI  (280)
1 site
T A C G T A A T G C A T
BtgZI  (274)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BmrI  (229)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
NdeI  (174)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
TaqII  (112)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
Start  (0)
0 sites
CMV enhancer
1 .. 304  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1 .. 304  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
305 .. 508  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
305 .. 508  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
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