Resources
Plasmid Files

GAL1 promoter

Yeast promoter with upstream activating sequence mediating Gal4-dependent induction.

 
To obtain this annotated DNA sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

 400 300 200 100 GAL1 promoter End (442) BstAPI (322) BtgZI (296) AseI (269) EarI (152) Bts α I (108) BsiEI (75) AgeI - BsaWI - BsrFI (70) NmeAIII (39) BseRI (38) BsrBI (19) Start (0) GAL1 promoter 442 bp
End  (442)
0 sites
BstAPI  (322)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BtgZI  (296)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AseI  (269)
1 site
A T T A A T T A A T T A
EarI  (152)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BtsαI  (108)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BsiEI  (75)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
AgeI  (70)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsaWI  (70)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BsrFI  (70)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
NmeAIII  (39)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BseRI  (38)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BsrBI  (19)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
Start  (0)
0 sites
GAL1 promoter
1 .. 442  =  442 bp
inducible promoter, regulated by Gal4
GAL1 promoter
1 .. 442  =  442 bp
inducible promoter, regulated by Gal4
UAS
1 .. 118  =  118 bp
upstream activating sequence mediating
Gal4-dependent induction
UAS
1 .. 118  =  118 bp
upstream activating sequence mediating
Gal4-dependent induction
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter