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Plasmid Files

HSV TK promoter

Herpes simplex virus (HSV) thymidine kinase promoter.

 
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 600 400 200 HSV TK promoter End (753) AflIII - MluI (707) BstBI (647) ApoI - EcoRI (643) NspI (593) PvuII (525) AcuI (493) BsaWI (382) BsaI (380) BpmI (373) AlwNI (351) BsaAI (340) BstZ17I (337) AccI (336) NcoI (324) AvrII (316) EcoO109I - PpuMI (313) BanII (307) SacII (293) PluTI (287) SfoI (285) BsaHI - NarI (284) BanI - KasI (283) SmaI (278) TspMI - XmaI (276) TaqII (248) BssHII (235) BspQI - EarI - SapI (131) MscI (122) EaeI (120) TsoI (29) Start (0) HSV TK promoter 753 bp
End  (753)
0 sites
AflIII  (707)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (707)
1 site
A C G C G T T G C G C A
BstBI  (647)
1 site
T T C G A A A A G C T T
ApoI  (643)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (643)
1 site
G A A T T C C T T A A G
NspI  (593)
1 site
R C A T G Y Y G T A C R
PvuII  (525)
1 site
C A G C T G G T C G A C
AcuI  (493)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaWI  (382)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BsaI  (380)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (373)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AlwNI  (351)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BsaAI  (340)
1 site
Y A C G T R R T G C A Y
BstZ17I  (337)
1 site
G T A T A C C A T A T G
AccI  (336)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NcoI  (324)
1 site
C C A T G G G G T A C C
AvrII  (316)
1 site
C C T A G G G G A T C C
EcoO109I  (313)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (313)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BanII  (307)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacII  (293)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PluTI  (287)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (285)
1 site
G G C G C C C C G C G G
BsaHI  (284)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (284)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
BanI  (283)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
KasI  (283)
1 site
G G C G C C C C G C G G
SmaI  (278)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (276)
1 site
C C C G G G G G G C C C
XmaI  (276)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
TaqII  (248)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BssHII  (235)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BspQI  (131)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (131)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (131)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
MscI  (122)
1 site
T G G C C A A C C G G T
EaeI  (120)
1 site
Y G G C C R R C C G G Y
TsoI  (29)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Start  (0)
0 sites
HSV TK promoter
1 .. 753  =  753 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
1 .. 753  =  753 bp
herpes simplex virus thymidine kinase promoter
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