Resources
Plasmid Files

LITMUS29

High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. The MCS is reversed relative to LITMUS28.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

LITMUS29.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
Download Free Trial Get SnapGene Viewer


PvuII (2707) AflII (2600) StuI (2598) BglII (2578) BsmI - NsiI (2574) BssHII (2562) BsiWI (2555) PaeR7I - XhoI (2547) EcoRI (2541) PstI - SbfI (2539) EcoRV (2531) BamHI (2521) HindIII (2515) BsmBI - BtgI - NcoI (2507) AatII (2503) ZraI (2501) AgeI (2493) XbaI (2487) AvrII (2480) SacI (2478) Eco53kI (2476) KpnI (2471) Acc65I (2467) SpeI (2460) SnaBI (2447) NspI (2313) AflIII - PciI (2309) PspFI (2009) BseYI (2005) AlwNI (1900) HpaI (3) TatI (447) ScaI (449) AseI (755) NmeAIII (783) BpmI (861) SwaI (1102) PsiI (1199) DraIII (1327) BtgZI (1328) LITMUS29 2820 bp
PvuII  (2707)
1 site
C A G C T G G T C G A C
AflII  (2600)
1 site
C T T A A G G A A T T C
StuI  (2598)
1 site
A G G C C T T C C G G A
BglII  (2578)
1 site
A G A T C T T C T A G A
BsmI  (2574)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NsiI  (2574)
1 site
A T G C A T T A C G T A
BssHII  (2562)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BsiWI  (2555)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
PaeR7I  (2547)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2547)
1 site
C T C G A G G A G C T C
EcoRI  (2541)
1 site
G A A T T C C T T A A G
PstI  (2539)
1 site
C T G C A G G A C G T C
SbfI  (2539)
1 site
C C T G C A G G G G A C G T C C
EcoRV  (2531)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BamHI  (2521)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
HindIII  (2515)
1 site
A A G C T T T T C G A A
BsmBI  (2507)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BtgI  (2507)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (2507)
1 site
C C A T G G G G T A C C
AatII  (2503)
1 site
G A C G T C C T G C A G
ZraI  (2501)
1 site
G A C G T C C T G C A G
AgeI  (2493)
1 site
A C C G G T T G G C C A
XbaI  (2487)
1 site
T C T A G A A G A T C T
AvrII  (2480)
1 site
C C T A G G G G A T C C