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LITMUS39

High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. The MCS is reversed relative to LITMUS38.

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LITMUS39.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
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PvuII (2704) AflII (2597) StuI (2595) ApaI (2582) EcoO109I (2579) PspOMI (2578) MfeI (2569) NaeI (2564) NgoMIV (2562) PluTI (2558) SfoI (2556) NarI * (2555) KasI (2554) HindIII (2545) PstI - SbfI (2542) EcoRV (2534) BamHI (2524) EcoRI (2517) BmtI (2515) NheI (2511) EagI (2504) BbsI (2500) MluI (2497) BspEI (2489) BsrGI (2482) SphI (2479) AccI (2469) SalI (2468) SpeI (2460) SnaBI (2447) PciI (2309) PspFI (2009) BseYI (2005) AlwNI (1900) HpaI (3) ScaI (449) AseI (755) NmeAIII (783) BpmI (861) BsaI (864) SwaI (1102) PsiI (1199) AvaI - BsoBI (1216) BmeT110I (1217) DraIII (1327) BtgZI (1328) LITMUS39 2817 bp
PvuII  (2704)
1 site
C A G C T G G T C G A C
AflII  (2597)
1 site
C T T A A G G A A T T C
StuI  (2595)
1 site
A G G C C T T C C G G A
ApaI  (2582)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
EcoO109I  (2579)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (2578)
1 site
G G G C C C C C C G G G
MfeI  (2569)
1 site
C A A T T G G T T A A C
NaeI  (2564)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2562)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PluTI  (2558)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2556)
1 site
G G C G C C C C G C G G
NarI  (2555)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2554)
1 site
G G C G C C C C G C G G
HindIII  (2545)
1 site
A A G C T T T T C G A A
PstI  (2542)
1 site
C T G C A G G A C G T C
SbfI  (2542)
1 site
C C T G C A G G G G A C G T C C
EcoRV  (2534)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BamHI  (2524)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2517)
1 site
G A A T T C C T T A A G