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Plasmid Files

PGK promoter

Mouse phosphoglycerate kinase 1 promoter.

 
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 400 300 200 100 PGK promoter End (500) EarI (482) BsmI (432) BspEI (420) EcoO109I - PpuMI (415) AlwNI (350) EaeI (308) BglI (301) StuI (295) BsrDI (285) BlpI (274) BssS α I (252) BsmBI - SpeI (246) AgeI (111) BaeGI - Bme1580I (63) BpmI - Eco57MI (52) NspI - SphI (40) StyI (21) Start (0) PGK promoter 500 bp
End  (500)
0 sites
EarI  (482)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BsmI  (432)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BspEI  (420)
1 site
T C C G G A A G G C C T
EcoO109I  (415)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (415)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
AlwNI  (350)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
EaeI  (308)
1 site
Y G G C C R R C C G G Y
BglI  (301)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
StuI  (295)
1 site
A G G C C T T C C G G A
BsrDI  (285)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BlpI  (274)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BssSαI  (252)
1 site
C A C G A G G T G C T C
BsmBI  (246)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SpeI  (246)
1 site
A C T A G T T G A T C A
AgeI  (111)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BaeGI  (63)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (63)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BpmI  (52)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (52)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NspI  (40)
1 site
R C A T G Y Y G T A C R
SphI  (40)
1 site
G C A T G C C G T A C G
StyI  (21)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
Start  (0)
0 sites
PGK promoter
1 .. 500  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
1 .. 500  =  500 bp
mouse phosphoglycerate kinase 1 promoter
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