SNAP-tag

Human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives.

Sequence Author: New England Biolabs

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500 400 300 200 100 End (546) BssSI - BssSαI (511) BanII (485) BmgBI (462) BaeGI - Bme1580I (419) BsrBI (404) Bpu10I (400) NaeI (363) BsrFI - NgoMIV (361) SfiI (358) SfcI (339) BseRI (244) Bsu36I (218) EciI (176) BtgI (128) BsaHI (125) EcoO109I (78) Start (0) SNAP-tag® SNAP-tag® 546 bp
End  (546)
0 sites
BssSI  (511)
1 site
C A C G A G G T G C T C
BssSαI  (511)
1 site
C A C G A G G T G C T C
BanII  (485)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BmgBI  (462)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BaeGI  (419)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (419)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsrBI  (404)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Bpu10I  (400)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NaeI  (363)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsrFI  (361)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NgoMIV  (361)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SfiI  (358)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SfcI  (339)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BseRI  (244)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Bsu36I  (218)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EciI  (176)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BtgI  (128)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaHI  (125)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EcoO109I  (78)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
Start  (0)
0 sites
SNAP-tag®
1 .. 546  =  546 bp
182 amino acids  =  19.4 kDa
Product: human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives
fast-reacting variant, optimized for mammalian expression
SNAP-tag®
1 .. 546  =  546 bp
182 amino acids  =  19.4 kDa
Product: human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives
fast-reacting variant, optimized for mammalian expression
ORF:  1 .. 546  =  546 bp
ORF:  182 amino acids  =  19.4 kDa
ORF:  1 .. 546  =  546 bp
ORF:  182 amino acids  =  19.4 kDa  (no start codon)
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Download SNAP-tag.dna file

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Individual Sequences & Maps

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