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pE-SUMOpro3 Kan

Bacterial vector with a kanamycin resistance marker, for regulated expression of recombinant proteins with a cleavable 6xHis-SUMO3 tag.

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pE-SUMOpro3 Kan.dna
Map and Sequence File:    Download    Open   
Sequence Author:  LifeSensors
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SgrAI (5570) SphI (5422) BstAPI (5213) MluI (4889) BclI * (4875) BstEII (4707) NmeAIII (4689) ApaI (4686) PspOMI (4682) BssHII (4478) EcoRV (4443) HpaI (4387) PshAI (4048) BglI (3832) FspI - FspAI (3811) PflFI - Tth111I (3046) BstZ17I (3021) TatI (2984) BspQI - SapI (2905) BglII (1) XbaI (67) NcoI (106) KflI (133) PstI - SbfI (142) BseRI (159) StuI (270) BmgBI (389) BsaI (409) SpeI (421) Acc65I (427) AgeI (430) KpnI (431) BsaI (439) BamHI (445) EcoRI (451) Eco53kI (459) SacI (461) SalI (464) HindIII (470) EagI - NotI (477) PaeR7I - PspXI - XhoI (485) DraIII (892) PsiI (1017) AsiSI - PvuI (1592) TspMI - XmaI (1714) SmaI (1716) BspDI - ClaI (1897) NruI (1933) AlwNI (2379) BssS α I (2615) PciI (2788) pE-SUMOpro3 Kan 5610 bp
SgrAI  (5570)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (5422)
1 site
G C A T G C C G T A C G
BstAPI  (5213)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (4889)
1 site
A C G C G T T G C G C A
BclI  (4875)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (4707)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (4689)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (4686)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (4682)
1 site
G G G C C C C C C G G G
BssHII  (4478)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoRV  (4443)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HpaI  (4387)
1 site
G T T A A C C A A T T G
PshAI  (4048)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BglI  (3832)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (3811)
1 site
T G C G C A A C G C G T
FspAI  (3811)
1 site
R T G C G C A Y Y A C G C G T R
PflFI  (3046)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3046)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstZ17I  (3021)
1 site
G T A T A C C A T A T G
TatI  (2984)
1 site
W G T A C W W C A T G W
BspQI  (2905)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2905)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BglII  (1)
1 site
A G A T C T T C T A G A
XbaI  (67)
1 site
T C T A G A A G A T C T
NcoI  (106)
1 site
C C A T G G G G T A C C
KflI  (133)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PstI  (142)
1 site
C T G C A G G A C G T C
SbfI  (142)
1 site
C C T G C A G G G G A C G T C C
BseRI  (159)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (270)
1 site
A G G C C T T C C G G A
BmgBI  (389)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BsaI  (409)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
SpeI  (421)
1 site
A C T A G T T G A T C A
Acc65I  (427)
1 site
G G T A C C C C A T G G
AgeI  (430)
1 site
A C C G G T T G G C C A
KpnI  (431)
1 site
G G T A C C C C A T G G
BsaI  (439)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BamHI  (445)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (451)
1 site
G A A T T C C T T A A G
Eco53kI  (459)
1 site
G A G C T C C T C G A G
SacI  (461)
1 site
G A G C T C C T C G A G
SalI  (464)
1 site
G T C G A C C A G C T G
HindIII  (470)
1 site
A A G C T T T T C G A A
EagI  (477)
1 site
C G G C C G G C C G G C
NotI  (477)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (485)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (485)
1 site
V C T C G A G B B G A G C T C V
XhoI  (485)
1 site
C T C G A G G A G C T C
DraIII  (892)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (1017)
1 site
T T A T A A A A T A T T
AsiSI  (1592)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1592)
1 site
C G A T C G G C T A G C
TspMI  (1714)
1 site
C C C G G G G G G C C C
XmaI  (1714)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1716)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BspDI  (1897)
1 site
A T C G A T T A G C T A
ClaI  (1897)
1 site
A T C G A T T A G C T A
NruI  (1933)
1 site
T C G C G A A G C G C T
AlwNI  (2379)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (2615)
1 site
C A C G A G G T G C T C
PciI  (2788)
1 site
A C A T G T T G T A C