pEX-N-His

PrecisionShuttle™ bacterial vector for inducible expression of a protein with a cleavable N-terminal 6xHis tag.

Sequence Author: OriGene

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
AanI - PsiI (4467) DraIII (4342) BsaAI (4339) XmnI (3789) ScaI (3670) TatI (3668) BsaI (3251) AhdI (3190) AlwNI (2713) EcoRI (179) T7 promoter lac operator RBS ATG NsiI (292) 6xHis TEV Site AsiSI - SgfI (334) MreI - SgrAI (336) AscI (340) BglII (347) HindIII (354) NheI (365) BmtI (369) RsrII (371) MluI (377) NotI (389) AvaI - BmeT110I - BsoBI - PaeR7I - PspXI - XhoI (395) PmeI (404) FseI (414) BlpI (444) StyI (466) EcoO109I (471) BtgI (522) SacII (525) PflMI (757) BstAPI (858) BclI * (1189) BstEII (1356) PspOMI (1382) ApaI (1386) EcoRV (1625) HincII - HpaI (1681) BsmBI (1790) KasI (1814) NarI * (1815) SfoI (1816) PluTI (1818) BamHI (2168) pEX-N-His 4539 bp
AanI  (4467)
1 site
T T A T A A A A T A T T
PsiI  (4467)
1 site
T T A T A A A A T A T T
DraIII  (4342)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaAI  (4339)
1 site
Y A C G T R R T G C A Y
XmnI  (3789)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3670)
1 site
A G T A C T T C A T G A
TatI  (3668)
1 site
W G T A C W W C A T G W
BsaI  (3251)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3190)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2713)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
EcoRI  (179)
1 site
G A A T T C C T T A A G
NsiI  (292)
1 site
A T G C A T T A C G T A
AsiSI  (334)
1 site
G C G A T C G C C G C T A G C G
SgfI  (334)
1 site
G C G A T C G C C G C T A G C G
MreI  (336)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (336)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (340)
1 site
G G C G C G C C C C G C G C G G
BglII  (347)
1 site
A G A T C T T C T A G A
HindIII  (354)
1 site
A A G C T T T T C G A A
NheI  (365)
1 site
G C T A G C C G A T C G
BmtI  (369)
1 site
G C T A G C C G A T C G
RsrII  (371)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (377)
1 site
A C G C G T T G C G C A
NotI  (389)
1 site
G C G G C C G C C G C C G G C G
AvaI  (395)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (395)
1 site
C Y C G R G G R G C Y C
BsoBI  (395)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (395)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (395)
1 site
V C T C G A G B B G A G C T C V
XhoI  (395)
1 site
C T C G A G G A G C T C
PmeI  (404)
1 site
G T T T A A A C C A A A T T T G
FseI  (414)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BlpI  (444)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
StyI  (466)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EcoO109I  (471)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BtgI  (522)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
SacII  (525)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PflMI  (757)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BstAPI  (858)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BclI  (1189)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (1356)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1382)
1 site
G G G C C C C C C G G G
ApaI  (1386)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
EcoRV  (1625)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HincII  (1681)
1 site
G T Y R A C C A R Y T G
HpaI  (1681)
1 site
G T T A A C C A A T T G
BsmBI  (1790)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
KasI  (1814)
1 site
G G C G C C C C G C G G
NarI  (1815)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1816)
1 site
G G C G C C C C G C G G
PluTI  (1818)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BamHI  (2168)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
lacI
825 .. 1907  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
825 .. 1907  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
3117 .. 3977  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3117 .. 3908  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3117 .. 3977  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3909 .. 3977  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3117 .. 3977  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2358 .. 2946  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2358 .. 2946  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4109 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4109 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
3978 .. 4082  =  105 bp
AmpR promoter
3978 .. 4082  =  105 bp
MCS
330 .. 416  =  87 bp
multiple cloning site
MCS
330 .. 416  =  87 bp
multiple cloning site
lacI promoter
747 .. 824  =  78 bp
lacI promoter
747 .. 824  =  78 bp
T7 terminator
455 .. 501  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
455 .. 501  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
ATG
288 .. 290  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
288 .. 290  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
291 .. 308  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
291 .. 308  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
TEV Site
309 .. 329  =  21 bp
7 amino acids  =  869.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV Site
309 .. 329  =  21 bp
7 amino acids  =  869.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
lac operator
230 .. 254  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
230 .. 254  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
211 .. 229  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
211 .. 229  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
274 .. 279  =  6 bp
ribosome binding site
RBS
274 .. 279  =  6 bp
ribosome binding site
ORF:  3247 .. 3513  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  758 .. 1075  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1664 .. 1927  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  2030 .. 2257  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  948 .. 1907  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  1690 .. 1941  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  3117 .. 3977  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pEX-N-His.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.