pEZ BAC

BAC vector with inducible copy number, suitable for cloning toxic inserts.

Sequence Author: Lucigen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
SacII (7159) FseI (7155) BciVI - MreI (7144) XcmI (6954) XbaI (6444) EcoNI (6170) SmaI (6147) TspMI - XmaI (6145) FspAI (5888) BsrGI (5856) SbfI (5619) BlpI (5158) AfeI (5074) MfeI (4649) NdeI (4633) BstXI (4559) KpnI (4433) Acc65I (4429) PflFI - Tth111I (4368) BseRI (3942) BclI * (3838) T3Te terminator AscI (120) NotI (148) BEZ-F1 (238 .. 261) BsrBI (278) lac operator HindIII (355) SphI (365) HpaI (379) BamHI (386) PmlI (398) EcoRI (403) BEZ-R1 (488 .. 511) KasI (590) NarI (591) SfoI (592) BbeI (594) NotI (620) PmeI (669) tonB terminator EcoO109I - PpuMI (983) DraIII (1024) ScaI (1918) BstAPI (1998) BstEII (2031) SexAI * (2035) PciI (2154) AhdI (2155) PspOMI (2668) ApaI - BanII (2672) AccI (2744) PfoI * (2884) SpeI (2914) AleI (3097) pEZ™ BAC 7260 bp
SacII  (7159)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
FseI  (7155)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BciVI  (7144)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
MreI  (7144)
1 site
C G C C G G C G G C G G C C G C
XcmI  (6954)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
XbaI  (6444)
1 site
T C T A G A A G A T C T
EcoNI  (6170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (6147)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (6145)
1 site
C C C G G G G G G C C C
XmaI  (6145)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
FspAI  (5888)
1 site
R T G C G C A Y Y A C G C G T R
BsrGI  (5856)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SbfI  (5619)
1 site
C C T G C A G G G G A C G T C C
BlpI  (5158)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AfeI  (5074)
1 site
A G C G C T T C G C G A
MfeI  (4649)
1 site
C A A T T G G T T A A C
NdeI  (4633)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstXI  (4559)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
KpnI  (4433)
1 site
G G T A C C C C A T G G
Acc65I  (4429)
1 site
G G T A C C C C A T G G
PflFI  (4368)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4368)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BseRI  (3942)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BclI  (3838)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AscI  (120)
1 site
G G C G C G C C C C G C G C G G
NotI  (148)
2 sites
G C G G C C G C C G C C G G C G
BsrBI  (278)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
HindIII  (355)
1 site
A A G C T T T T C G A A
SphI  (365)
1 site
G C A T G C C G T A C G
HpaI  (379)
1 site
G T T A A C C A A T T G
BamHI  (386)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PmlI  (398)
1 site
C A C G T G G T G C A C
EcoRI  (403)
1 site
G A A T T C C T T A A G
KasI  (590)
1 site
G G C G C C C C G C G G
NarI  (591)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (592)
1 site
G G C G C C C C G C G G
BbeI  (594)
1 site
G G C G C C C C G C G G

Cleavage may be enhanced when more than one copy of the BbeI recognition sequence is present.
NotI  (620)
2 sites
G C G G C C G C C G C C G G C G
PmeI  (669)
1 site
G T T T A A A C C A A A T T T G
EcoO109I  (983)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (983)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
DraIII  (1024)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (1918)
1 site
A G T A C T T C A T G A
BstAPI  (1998)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BstEII  (2031)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
SexAI  (2035)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
PciI  (2154)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (2155)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspOMI  (2668)
1 site
G G G C C C C C C G G G
ApaI  (2672)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (2672)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
AccI  (2744)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PfoI  (2884)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SpeI  (2914)
1 site
A C T A G T T G A T C A
AleI  (3097)
1 site
C A C N N N N G T G G T G N N N N C A C
BEZ-F1
24-mer  /  50% GC
1 binding site
238 .. 261  =  24 annealed bases
Tm  =  60°C
BEZ-R1
24-mer  /  54% GC
1 binding site
488 .. 511  =  24 annealed bases
Tm  =  63°C
sopA
3735 .. 4910  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
3735 .. 4910  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
repE
5489 .. 6244  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
5489 .. 6244  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
CmR
1288 .. 1947  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1288 .. 1947  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6630 .. 7244  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6630 .. 7244  =  615 bp
origin of replication for the bacterial F plasmid
sopC
2218 .. 2691  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
2218 .. 2691  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
cos
764 .. 1162  =  399 bp
lambda cos site; allows packaging into phage lambda particles
cos
764 .. 1162  =  399 bp
lambda cos site; allows packaging into phage lambda particles
lacZα
308 .. 580  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
308 .. 580  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
incC
5236 .. 5486  =  251 bp
incompatibility region of the bacterial F plasmid
incC
5236 .. 5486  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
6335 .. 6554  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
6335 .. 6554  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
loxP
79 .. 112  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
79 .. 112  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
632 .. 665  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
632 .. 665  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
tonB terminator
684 .. 715  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
684 .. 715  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
lac promoter
234 .. 264  =  31 bp
3 segments
   Segment 1:  -35  
   234 .. 239  =  6 bp
promoter for the E. coli lac operon
lac promoter
234 .. 264  =  31 bp
3 segments
   Segment 2:  
   240 .. 257  =  18 bp
promoter for the E. coli lac operon
lac promoter
234 .. 264  =  31 bp
3 segments
   Segment 3:  -10  
   258 .. 264  =  7 bp
promoter for the E. coli lac operon
lac promoter
234 .. 264  =  31 bp
3 segments
promoter for the E. coli lac operon
T3Te terminator
28 .. 57  =  30 bp
phage T3 early transcription terminator
T3Te terminator
28 .. 57  =  30 bp
phage T3 early transcription terminator
T7Te terminator
1962 .. 1985  =  24 bp
phage T7 early transcription terminator
T7Te terminator
1962 .. 1985  =  24 bp
phage T7 early transcription terminator
lac operator
272 .. 288  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
272 .. 288  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
sopB
2764 .. 3735  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
2764 .. 3735  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
T7 promoter
411 .. 429  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
411 .. 429  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  1288 .. 1947  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  308 .. 580  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  3458 .. 3694  =  237 bp
ORF:  78 amino acids  =  8.3 kDa
ORF:  5855 .. 6088  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  363 .. 635  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
ORF:  2764 .. 3735  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  5695 .. 5940  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  6025 .. 6255  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  7107 .. 95  =  249 bp
ORF:  82 amino acids  =  8.8 kDa
ORF:  2985 .. 3383  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  3735 .. 4910  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
ORF:  5489 .. 6244  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
Click here to try SnapGene

Download pEZ BAC.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.