pFN2A (GST)

Flexi® vector with an ampicillin resistance marker, for bacterial or in vitro expression of a protein with a cleavable N-terminal GST tag.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
T7 promoter BglII (3689) MluI (3570) cer region DrdI (2701) AgeI (2525) AhdI (2423) BglI (2305) FspI (2200) EcoNI (80) BspQI - SapI (149) BtgZI (188) MscI (277) BsgI (356) BclI * (504) AsiSI - PvuI - SgfI (764) BtgI - NcoI (767) DraI - PmeI (1131) EcoRI (1136) Eco53kI (1144) BanII - SacI (1146) Acc65I (1148) AvaI - BmeT110I - BsoBI - KpnI - TspMI - XmaI (1152) SmaI (1154) BamHI (1157) XbaI (1163) SalI (1169) AccI (1170) HincII (1171) PstI - SbfI (1179) SphI (1185) BlpI (1244) BsaAI - SnaBI (1485) SspI (1618) pFN2A (GST) 4137 bp
BglII  (3689)
1 site
A G A T C T T C T A G A
MluI  (3570)
1 site
A C G C G T T G C G C A
DrdI  (2701)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AgeI  (2525)
1 site
A C C G G T T G G C C A
AhdI  (2423)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BglI  (2305)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (2200)
1 site
T G C G C A A C G C G T
EcoNI  (80)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BspQI  (149)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (149)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BtgZI  (188)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MscI  (277)
1 site
T G G C C A A C C G G T
BsgI  (356)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BclI  (504)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AsiSI  (764)
1 site
G C G A T C G C C G C T A G C G
PvuI  (764)
1 site
C G A T C G G C T A G C
SgfI  (764)
1 site
G C G A T C G C C G C T A G C G
BtgI  (767)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (767)
1 site
C C A T G G G G T A C C
DraI  (1131)
1 site
T T T A A A A A A T T T
PmeI  (1131)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (1136)
1 site
G A A T T C C T T A A G
Eco53kI  (1144)
1 site
G A G C T C C T C G A G
BanII  (1146)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (1146)
1 site
G A G C T C C T C G A G
Acc65I  (1148)
1 site
G G T A C C C C A T G G
AvaI  (1152)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (1152)
1 site
C Y C G R G G R G C Y C
BsoBI  (1152)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
KpnI  (1152)
1 site
G G T A C C C C A T G G
TspMI  (1152)
1 site
C C C G G G G G G C C C
XmaI  (1152)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1154)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1157)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1163)
1 site
T C T A G A A G A T C T
SalI  (1169)
1 site
G T C G A C C A G C T G
AccI  (1170)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (1171)
1 site
G T Y R A C C A R Y T G
PstI  (1179)
1 site
C T G C A G G A C G T C
SbfI  (1179)
1 site
C C T G C A G G G G A C G T C C
SphI  (1185)
1 site
G C A T G C C G T A C G
BlpI  (1244)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsaAI  (1485)
1 site
Y A C G T R R T G C A Y
SnaBI  (1485)
1 site
T A C G T A A T G C A T
SspI  (1618)
1 site
A A T A T T T T A T A A
AmpR
1636 .. 2496  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1636 .. 1704  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1636 .. 2496  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1705 .. 2496  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1636 .. 2496  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
GST
70 .. 723  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from Schistosoma japonicum
GST
70 .. 723  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from Schistosoma japonicum
TEV site
742 .. 762  =  21 bp
7 amino acids  =  884.0 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV site
742 .. 762  =  21 bp
7 amino acids  =  884.0 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
ori
2654 .. 3242  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2654 .. 3242  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
791 .. 1126  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
791 .. 1126  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
3358 .. 3641  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
3358 .. 3641  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
AmpR promoter
1531 .. 1635  =  105 bp
AmpR promoter
1531 .. 1635  =  105 bp
rrnB T1 terminator
3871 .. 3957  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3871 .. 3957  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T7 terminator
1255 .. 1302  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
1255 .. 1302  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
rrnB T2 terminator
4049 .. 4076  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
4049 .. 4076  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  70 .. 777  =  708 bp
ORF:  235 amino acids  =  27.2 kDa
ORF:  1636 .. 2496  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  791 .. 1126  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  669 .. 1079  =  411 bp
ORF:  136 amino acids  =  15.5 kDa
ORF:  3603 .. 3950  =  348 bp
ORF:  115 amino acids  =  12.4 kDa
ORF:  3712 .. 4038  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  507 .. 770  =  264 bp
ORF:  87 amino acids  =  9.7 kDa
ORF:  2100 .. 2366  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  623 .. 847  =  225 bp
ORF:  74 amino acids  =  8.6 kDa
Click here to try SnapGene

Download pFN2A (GST).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.