pFN6A (HQ)

Flexi® vector with an ampicillin resistance marker, for bacterial or in vitro expression of a protein with an N-terminal HQ metal affinity tag.
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
T7 promoter BglII (3023) MluI (2904) BspEI (2691) DrdI (2035) PciI (1927) AgeI (1859) BstBI (1832) AhdI (1757) ATG HQ tag AsiSI - PvuI - SgfI (98) BtgI - NcoI (101) DraI - PmeI (465) BanII (480) Acc65I (482) KpnI (486) BamHI (491) XbaI (497) SalI (503) AccI (504) HincII (505) PstI - SbfI (513) SphI (519) BlpI (578) EcoO109I (605) BsaAI - SnaBI (819) SspI (952) EarI (958) XmnI (1157) FspI (1534) BglI (1639) BpmI (1688) pFN6A (HQ) 3471 bp
BglII  (3023)
1 site
A G A T C T T C T A G A
MluI  (2904)
1 site
A C G C G T T G C G C A
BspEI  (2691)
1 site
T C C G G A A G G C C T
DrdI  (2035)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PciI  (1927)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (1859)
1 site
A C C G G T T G G C C A
BstBI  (1832)
1 site
T T C G A A A A G C T T
AhdI  (1757)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AsiSI  (98)
1 site
G C G A T C G C C G C T A G C G
PvuI  (98)
1 site
C G A T C G G C T A G C
SgfI  (98)
1 site
G C G A T C G C C G C T A G C G
BtgI  (101)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (101)
1 site
C C A T G G G G T A C C
DraI  (465)
1 site
T T T A A A A A A T T T
PmeI  (465)
1 site
G T T T A A A C C A A A T T T G
BanII  (480)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Acc65I  (482)
1 site
G G T A C C C C A T G G
KpnI  (486)
1 site
G G T A C C C C A T G G
BamHI  (491)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (497)
1 site
T C T A G A A G A T C T
SalI  (503)
1 site
G T C G A C C A G C T G
AccI  (504)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (505)
1 site
G T Y R A C C A R Y T G
PstI  (513)
1 site
C T G C A G G A C G T C
SbfI  (513)
1 site
C C T G C A G G G G A C G T C C
SphI  (519)
1 site
G C A T G C C G T A C G
BlpI  (578)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (605)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaAI  (819)
1 site
Y A C G T R R T G C A Y
SnaBI  (819)
1 site
T A C G T A A T G C A T
SspI  (952)
1 site
A A T A T T T T A T A A
EarI  (958)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
XmnI  (1157)
1 site
G A A N N N N T T C C T T N N N N A A G
FspI  (1534)
1 site
T G C G C A A C G C G T
BglI  (1639)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BpmI  (1688)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AmpR
970 .. 1830  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   970 .. 1038  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
970 .. 1830  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1039 .. 1830  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
970 .. 1830  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1988 .. 2576  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1988 .. 2576  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
125 .. 460  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
125 .. 460  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
2692 .. 2975  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
2692 .. 2975  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
AmpR promoter
865 .. 969  =  105 bp
AmpR promoter
865 .. 969  =  105 bp
rrnB T1 terminator
3205 .. 3291  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3205 .. 3291  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T7 terminator
589 .. 636  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
589 .. 636  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
rrnB T2 terminator
3383 .. 3410  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
3383 .. 3410  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
ATG
70 .. 72  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
70 .. 72  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
HQ tag
76 .. 93  =  18 bp
6 amino acids  =  813.8 Da
Product: HQHQHQ metal affinity tag
The HQ tag binds to nickel and other metal ions much like the 6xHis tag, but may allow elution at lower concentrations of imidazole (Godat et al., 2008).
HQ tag
76 .. 93  =  18 bp
6 amino acids  =  813.8 Da
Product: HQHQHQ metal affinity tag
The HQ tag binds to nickel and other metal ions much like the 6xHis tag, but may allow elution at lower concentrations of imidazole (Godat et al., 2008).
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  970 .. 1830  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  125 .. 460  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  2937 .. 3284  =  348 bp
ORF:  115 amino acids  =  12.4 kDa
ORF:  3046 .. 3372  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  1434 .. 1700  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pFN6A (HQ).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.