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pGS-21a

Bacterial vector for expression of 6xHis- and GST-tagged proteins. Two 6xHis sequences are encoded in the vector.

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pGS-21a.dna
Map and Sequence File:    Download    Open   
Sequence Author:  GenScript
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BspDI * - ClaI * (6149) SgrAI (6105) SphI (5957) PflMI (5849) BstAPI (5748) MluI (5424) BstEII (5242) ApaI (5221) PspOMI (5217) BssHII (5013) HpaI (4922) PshAI (4583) FspAI (4346) PpuMI (4318) Bpu10I (4218) PflFI - Tth111I (3581) BstZ17I (3556) T7 primer (1 .. 20) XbaI (47) NdeI (87) MscI (335) BstBI (525) SwaI (555) SfiI (798) BglII (806) Acc65I (812) KpnI (816) NcoI (834) EcoRV (844) BamHI (848) EcoRI (854) Eco53kI (862) SacI (864) SalI (866) HindIII (872) EagI - NotI (879) AvaI - BsoBI - PaeR7I - PspXI - XhoI (887) BmeT110I (888) BlpI (966) T7 terminator primer (963 .. 981) DraIII (1294) PsiI (1419) PvuI (2066) PstI (2193) BsaI (2369) AhdI (2435) AlwNI (2914) pGS-21a 6169 bp
BspDI  (6149)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (6149)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SgrAI  (6105)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (5957)
1 site
G C A T G C C G T A C G
PflMI  (5849)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BstAPI  (5748)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (5424)
1 site
A C G C G T T G C G C A
BstEII  (5242)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaI  (5221)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (5217)
1 site
G G G C C C C C C G G G
BssHII  (5013)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HpaI  (4922)
1 site
G T T A A C C A A T T G
PshAI  (4583)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
FspAI  (4346)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (4318)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Bpu10I  (4218)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflFI  (3581)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3581)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstZ17I  (3556)
1 site
G T A T A C C A T A T G
XbaI  (47)
1 site
T C T A G A A G A T C T
NdeI  (87)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
MscI  (335)
1 site
T G G C C A A C C G G T
BstBI  (525)
1 site
T T C G A A A A G C T T
SwaI  (555)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
SfiI  (798)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BglII  (806)
1 site
A G A T C T T C T A G A
Acc65I  (812)
1 site
G G T A C C C C A T G G
KpnI  (816)
1 site
G G T A C C C C A T G G
NcoI  (834)
1 site
C C A T G G G G T A C C
EcoRV  (844)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BamHI  (848)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (854)
1 site
G A A T T C C T T A A G
Eco53kI  (862)
1 site
G A G C T C C T C G A G
SacI  (864)
1 site
G A G C T C C T C G A G
SalI  (866)
1 site
G T C G A C C A G C T G
HindIII  (872)
1 site
A A G C T T T T C G A A
EagI  (879)
1 site
C G G C C G G C C G G C
NotI  (879)
1 site
G C G G C C G C C G C C G G C G
AvaI  (887)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (887)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (887)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (887)
1 site
V C T C G A G B B G A G C T C V
XhoI  (887)
1 site
C T C G A G G A G C T C
BmeT110I  (888)
1 site
C Y C G R G G R G C Y C
BlpI  (966)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
DraIII  (1294)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (1419)
1 site
T T A T A A A A T A T T
PvuI  (2066)
1 site
C G A T C G