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Plasmid Files

pJAZZ®-OK

Linear bacterial vector with a kanamycin resistance gene for cloning DNA sequences that are unstable in circular plasmids.

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pJAZZ-OK.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lucigen
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12,500 10,000 7500 5000 2500 telL CB lacZ α cat promoter KanR telR N15 ori End (13,042) PacI (12,546) BglII (11,936) tonB terminator AvrII (11,884) AsiSI (11,499) rrnB T1 terminator NZ RevC (10,877 .. 10,898) SwaI (10,898) BsrBI (10,494) BmtI (10,366) NheI (10,362) NaeI (10,355) NgoMIV (10,353) ApaI (10,324) PspOMI (10,320) SpeI (10,293) SL1 (10,272 .. 10,292) T7Te terminator XbaI (10,207) BssS α I (9891) RsrII (9656) BbvCI (8044) PluTI (7282) SfoI (7280) NarI (7279) KasI (7278) BsrGI (5170) AatII (4824) ZraI (4822) BstBI (4086) SexAI * (3996) MauBI (3125) SacI (1874) Eco53kI (1872) AarI (1785) PpuMI (1632) SgrDI (1402) BstZ17I (1064) PmeI (956) Start (0) pJAZZ®-OK 13,042 bp
End  (13,042)
0 sites
PacI  (12,546)
1 site
T T A A T T A A A A T T A A T T
BglII  (11,936)
1 site
A G A T C T T C T A G A
AvrII  (11,884)
1 site
C C T A G G G G A T C C
AsiSI  (11,499)
1 site
G C G A T C G C C G C T A G C G
SwaI  (10,898)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BsrBI  (10,494)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
BmtI  (10,366)
1 site
G C T A G C C G A T C G
NheI  (10,362)
1 site
G C T A G C C G A T C G
NaeI  (10,355)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (10,353)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
ApaI  (10,324)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (10,320)
1 site
G G G C C C C C C G G G
SpeI  (10,293)
1 site
A C T A G T T G A T C A
XbaI  (10,207)
1 site
T C T A G A A G A T C T
BssSαI  (9891)
1 site
C A C G A G G T G C T C
RsrII  (9656)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BbvCI  (8044)
1 site
C C T C A G C G G A G T C G
PluTI  (7282)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (7280)
1 site
G G C G C C C C G C G G
NarI  (7279)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (7278)
1 site
G G C G C C C C G C G G
BsrGI  (5170)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AatII  (4824)
1 site
G A C G T C C T G C A G
ZraI  (4822)
1 site
G A C G T C C T G C A G
BstBI  (4086)
1 site
T T C G A A A A G C T T
SexAI  (3996)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MauBI  (3125)
1 site
C G C G C G C G G C G C G C G C
SacI  (1874)
1 site
G A G C T C C T C G A G
Eco53kI  (1872)
1 site
G A G C T C C T C G A G
AarI  (1785)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PpuMI  (1632)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SgrDI  (1402)
1 site
C G T C G A C G G C A G C T G C
BstZ17I  (1064)
1 site
G T A T A C C A T A T G
PmeI  (956)
1 site
G T T T A A A C C A A A T T T G
Start  (0)
0 sites
NZ RevC
22-mer  /  32% GC
1 binding site
10,877 .. 10,898  =  22 annealed bases
Tm  =  52°C
sequencing primer
SL1
21-mer  /  57% GC
1 binding site
10,272 .. 10,292  =  21 annealed bases
Tm  =  59°C
sequencing primer
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
MCS-L
10,299 .. 10,336  =  38 bp
multiple cloning site, left side
MCS-L
10,299 .. 10,336  =  38 bp
multiple cloning site, left side
lac promoter
10,450 .. 10,480  =  31 bp
   Segment 1:  -35  
   10,450 .. 10,455  =  6 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
   Segment 2:  
   10,456 .. 10,473  =  18 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
   Segment 3:  -10  
   10,474 .. 10,480  =  7 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
3 segments
promoter for the E. coli lac operon
lacZα
10,524 .. 10,796  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
allows blue/white screening
lacZα
10,524 .. 10,796  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
allows blue/white screening
MCS-R
10,819 .. 10,851  =  33 bp
multiple cloning site, right side
MCS-R
10,819 .. 10,851  =  33 bp
multiple cloning site, right side
rrnB T1 terminator
10,917 .. 10,962  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
10,917 .. 10,962  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
cat promoter
10,966 .. 11,067  =  102 bp
promoter of the E. coli cat gene
cat promoter
10,966 .. 11,067  =  102 bp
promoter of the E. coli cat gene
KanR
11,068 .. 11,883  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
11,068 .. 11,883  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
tonB terminator
11,897 .. 11,928  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
11,897 .. 11,928  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
telR
13,015 .. 13,042  =  28 bp
telomeric sequence from coliphage N15
telR
13,015 .. 13,042  =  28 bp
telomeric sequence from coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
ORF:  193 .. 2088  =  1896 bp
ORF:  631 amino acids  =  72.3 kDa
ORF:  9997 .. 10,320  =  324 bp
ORF:  107 amino acids  =  11.9 kDa
ORF:  10,579 .. 10,878  =  300 bp
ORF:  99 amino acids  =  10.8 kDa
ORF:  11,068 .. 11,883  =  816 bp
ORF:  271 amino acids  =  30.9 kDa
ORF:  1313 .. 1651  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  3032 .. 3268  =  237 bp
ORF:  78 amino acids  =  9.0 kDa
ORF:  7082 .. 7555  =  474 bp
ORF:  157 amino acids  =  17.8 kDa
ORF:  2067 .. 2291  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  5169 .. 5426  =  258 bp
ORF:  85 amino acids  =  8.7 kDa
ORF:  5466 .. 5786  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  6684 .. 6917  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  7317 .. 7544  =  228 bp
ORF:  75 amino acids  =  8.2 kDa
ORF:  8127 .. 8402  =  276 bp
ORF:  91 amino acids  =  10.0 kDa
ORF:  10,524 .. 10,796  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  3053 .. 3283  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  3812 .. 4252  =  441 bp
ORF:  146 amino acids  =  17.5 kDa
ORF:  5084 .. 9058  =  3975 bp
ORF:  1324 amino acids  =  149.5 kDa
ORF:  10,643 .. 10,876  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  1300 .. 1605  =  306 bp
ORF:  101 amino acids  =  11.1 kDa
ORF:  1639 .. 1926  =  288 bp
ORF:  95 amino acids  =  10.9 kDa
ORF:  3280 .. 3582  =  303 bp
ORF:  100 amino acids  =  10.9 kDa
ORF:  4165 .. 4473  =  309 bp
ORF:  102 amino acids  =  11.7 kDa
ORF:  4498 .. 4827  =  330 bp
ORF:  109 amino acids  =  12.6 kDa
ORF:  6439 .. 6696  =  258 bp
ORF:  85 amino acids  =  9.2 kDa
ORF:  9307 .. 9915  =  609 bp
ORF:  202 amino acids  =  22.7 kDa
ORF:  11,899 .. 12,600  =  702 bp
ORF:  233 amino acids  =  26.0 kDa
ORF:  1464 .. 1817  =  354 bp
ORF:  117 amino acids  =  12.3 kDa
ORF:  2262 .. 3056  =  795 bp
ORF:  264 amino acids  =  29.7 kDa
ORF:  4824 .. 5072  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
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