Resources
Plasmid Files

pMAL-c5X

Bacterial vector for inducible cytoplasmic expression of maltose-binding protein (MBP) fusions with a Factor Xa cleavage site.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pMAL-c5X.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
Download Free Trial Get SnapGene Viewer


MscI * (5652) FspAI (5642) Bpu10I (5514) PflFI - Tth111I (4877) BspQI - SapI (4796) PciI (4679) Bsu36I (3990) BglI (3756) ScaI (3393) TatI (3391) DraI (3296) BspHI (3034) RsrII (2990) PflMI (13) MluI (431) BstEII (612) PspOMI (638) ApaI (642) HpaI (937) KasI (1070) NarI * (1071) SfoI (1072) PluTI (1074) MfeI (1502) BsiWI (1818) PsiI (1880) BglII (1887) BmgBI (2070) BsmI (2148) BlpI (2314) Eco53kI (2636) SacI (2638) AvaI - BsoBI (2671) BmeT110I (2672) XmnI (2688) NdeI (2696) NcoI - StyI (2702) EagI - NotI (2709) EcoRV (2718) SalI - SgrDI (2722) AccI (2723) BamHI (2728) EcoRI (2734) PstI - SbfI (2745) HindIII (2757) pMAL-c5X 5677 bp
MscI  (5652)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
FspAI  (5642)
1 site
R T G C G C A Y Y A C G C G T R
Bpu10I  (5514)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflFI  (4877)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4877)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BspQI  (4796)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4796)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (4679)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
Bsu36I  (3990)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BglI  (3756)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
ScaI  (3393)
1 site
A G T A C T T C A T G A
TatI  (3391)
1 site
W G T A C W W C A T G W
DraI  (3296)
1 site
T T T A A A A A A T T T
BspHI  (3034)
1 site
T C A T G A A G T A C T
RsrII  (2990)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflMI  (13)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
MluI  (431)
1 site
A C G C G T T G C G C A
BstEII  (612)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (638)
1 site
G G G C C C C C C G G G
ApaI  (642)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
HpaI  (937)
1 site
G T T A A C C A A T T G
KasI  (1070)
1 site
G G C G C C C C G C G G
NarI  (1071)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1072)
1 site
G G C G C C C C G C G G
PluTI  (1074)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
MfeI  (1502)
1 site
C A A T T G G T T A A C
BsiWI  (1818)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
PsiI  (1880)
1 site
T T A T A A A A T A T T
BglII  (1887)
1 site
A G A T C T T C T A G A
BmgBI  (2070)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BsmI  (2148)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BlpI  (2314)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Eco53kI  (2636)
1 site
G A G C T C C T C G A G
SacI  (2638)
1 site