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Plasmid Files

pMMB206

Broad-host-range vector with a chloramphenicol resistance gene, suitable for blue-white screening and expression of toxic proteins.

 
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pMMB206.dna
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TspMI - XmaI (3) EcoRI (9311) MauBI (8935) MluI (8478) ApaI - BanII (8275) PspOMI (8271) HpaI (7976) BspQI - SapI (7670) FspAI (7199) EcoNI (7049) PfoI * (6909) SgrAI (4961) SmaI (5) BamHI (8) SalI (14) SfcI (20) PstI (24) HindIII (28) PvuI (154) XmnI (215) BsaI (363) BssS α I (827) BspEI (1471) SnaBI (1481) SfiI (2101) EcoO109I (2653) BstZ17I (3476) pMMB206 9313 bp
TspMI  (3)
1 site
C C C G G G G G G C C C
XmaI  (3)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
EcoRI  (9311)
1 site
G A A T T C C T T A A G
MauBI  (8935)
1 site
C G C G C G C G G C G C G C G C
MluI  (8478)
1 site
A C G C G T T G C G C A
ApaI  (8275)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (8275)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (8271)
1 site
G G G C C C C