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pSTBlue-1

Multi-purpose bacterial cloning and expression vector with a versatile MCS, T7 and SP6 promoters, and ampicillin and kanamycin resistance genes.

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pSTBlue-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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KpnI (55) Acc65I (51) BspQI - SapI (3627) PciI (3510) PspFI (3210) BseYI (3206) AlwNI (3101) PflMI (2660) Bpu10I (2414) BsmBI (2413) AsiSI (2397) EcoNI (2309) SmaI (2271) TspMI - XmaI (2269) BspDI - ClaI (2088) NruI (2054) SphI (63) PstI (68) MluI (70) SnaBI (78) BamHI (84) EcoRI (91) EcoRV (101) EcoRI (106) SalI (112) AccI (113) HincII (114) HindIII (118) PaeR7I - XhoI (124) AvrII - StyI (130) NheI (135) BmtI (139) XbaI (141) AleI - PmlI (152) BstXI (154) EcoO109I - PspOMI (159) ApaI (163) Eco53kI (167) SacI (169) EagI - NotI (172) AanI - PsiI (469) DraIII (597) BtgZI (598) NgoMIV (698) NaeI (700) BsaHI (1207) TatI (1264) ScaI (1266) NmeAIII (1600) BpmI (1678) BsaI (1681) AhdI (1747) pSTBlue-1 3851 bp
KpnI  (55)
1 site
G G T A C C C C A T G G
Acc65I  (51)
1 site
G G T A C C C C A T G G
BspQI  (3627)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3627)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (3510)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (3210)
1 site
C C C A G C G G G T C G
BseYI  (3206)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (3101)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PflMI  (2660)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Bpu10I  (2414)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2413)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AsiSI  (2397)
1 site
G C G A T C G C C G C T A G C G
EcoNI  (2309)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (2271)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (2269)
1 site
C C C G G G G G G C C C
XmaI  (2269)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BspDI  (2088)
1 site
A T C G A T T A G C T A
ClaI  (2088)
1 site
A T C G A T T A G C T A
NruI  (2054)
1 site
T C G C G A A G C G C T
SphI  (63)
1 site
G C A T G C C G T A C G
PstI  (68)
1 site
C T G C A G G