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Plasmid Files

pT7CFE1-NHis-GST-CHA

Vector with N-terminal cleavable 9xHis and GST tags and a C-terminal HA tag, for use in a human in vitro protein expression system.

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pT7CFE1-NHis-GST-CHA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Pierce)
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PvuII (4284) NaeI - PdiI (4082) MroNI - NgoMIV (4080) AanI - PsiI (3851) PfoI (3562) AatII (3451) ZraI (3449) NmeAIII (2677) BpmI - GsuI (2599) BsaI - Bso31I - BspTNI - Eco31I (2590) AhdI - AspEI - BmeRI - DriI - Eam1105I (2529) AspA2I - AvrII - BlnI - XmaJI (110) HindIII (183) AcvI - BbrPI - Eco72I - PmaCI - PmlI - PspCI (275) AarI - Acc36I - BfuAI - BspMI - BveI (298) Acc65I - Asp718I (400) KpnI (404) AccB7I - PflMI - Van91I (412) BstDSI - BtgI (492) BstENI - EcoNI - XagI (582) BspQI - LguI - PciSI - SapI (651) BsgI (858) AsuII - Bpu14I - Bsp119I - BspT104I - BstBI - Csp45I - NspV - SfuI (969) SmiI - SwaI (999) BclI * - FbaI * - Ksp22I * (1006) FauNDI - NdeI (1287) BamHI (1292) EcoRI (1298) Eco32I - EcoRV (1306) PacI (1314) PstI (1323) Ecl136II - Eco53kI - EcoICRI (1327) Psp124BI - SacI - SstI (1329) SalI - SgrDI (1331) AccI - FblI - XmiI (1332) HincII - HindII (1333) BseX3I - BstZI - CciNI - EagI - EclXI - Eco52I - NotI (1338) Ama87I - AvaI - BsiHKCI - BsoBI - Eco88I - PaeR7I - PspXI - Sfr274I - SlaI - XhoI (1346) BmeT110I (1347) BglII (1381) MssI - PmeI (1425) AhlI - BcuI - SpeI (1430) BlpI - Bpu1102I - Bsp1720I - CelII (1439) BseYI (1940) GsaI - PspFI (1944) AlwNI - CaiI (2052)