Resources
Plasmid Files

pYES1L

Linearized BAC/YAC shuttle vector for assembling large DNA fragments using the GeneArt® High-Order Genetic Assembly System.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pYES1L.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
Download Free Trial Get SnapGene Viewer


8000 6000 4000 2000 TRP1 TRP1 promoter CEN/ARS AmpR promoter SmR ori2 incC sopC oriT End (9380) NotI (9360) I-SceI (9322) I-CeuI (59) PacI (16) Start (0) pYES1L 9380 bp
End  (9380)
0 sites
NotI  (9360)
1 site
G C G G C C G C C G C C G G C G
I-SceI  (9322)
1 site
T A G G G A T A A C A G G G T A A T A T C C C T A T T G T C C C A T T A

I-SceI is a homing endonuclease that can recognize a variety of similar recognition sequences.
After cleavage, I-SceI can remain bound to DNA and alter its electrophoretic mobility.
I-CeuI  (59)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
PacI  (16)
1 site
T T A A T T A A A A T T A A T T
Start  (0)
0 sites
TRP1
172 .. 846  =  675 bp
224 amino acids  =  24.2 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1
172 .. 846  =  675 bp
224 amino acids  =  24.2 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1 promoter
847 .. 1127  =  281 bp
TRP1 promoter
847 .. 1127  =  281 bp
CEN/ARS
1386 .. 1889  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
CEN/ARS
1386 .. 1889  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
AmpR promoter
1926 .. 2030  =  105 bp
AmpR promoter
1926 .. 2030  =  105 bp
SmR
2514 .. 3305  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
2514 .. 3305  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori2
4367 .. 4586  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
4367 .. 4586  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
repE
4677 .. 5432  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
4677 .. 5432  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
incC
5435 .. 5685  =  251 bp
incompatibility region of the bacterial F plasmid
incC
5435 .. 5685  =  251 bp
incompatibility region of the bacterial F plasmid
sopA
6011 .. 7186  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
6011 .. 7186  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopC
8230 .. 8703  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
8230 .. 8703  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
oriT
8994 .. 9284  =  291 bp
origin of transfer for the bacterial F plasmid (Frost et al., 1994)
oriT
8994 .. 9284  =  291 bp
origin of transfer for the bacterial F plasmid (Frost et al., 1994)
sopB
7186 .. 8157  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
7186 .. 8157  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
ORF:  3556 .. 3903  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  4666 .. 4896  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  4981 .. 5226  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  7186 .. 8157  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  74 .. 436  =  363 bp
ORF:  120 amino acids  =  13.3 kDa
ORF:  6011 .. 7186  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
ORF:  7538 .. 7936  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  2295 .. 3305  =  1011 bp
ORF:  336 amino acids  =  37.4 kDa
ORF:  4677 .. 5432  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
ORF:  6192 .. 6344  =  153 bp
ORF:  50 amino acids  =  6.0 kDa
ORF:  315 .. 596  =  282 bp
ORF:  93 amino acids  =  10.8 kDa
ORF:  2553 .. 2936  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  4833 .. 5066  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  7227 .. 7463  =  237 bp
ORF:  78 amino acids  =  8.2 kDa
ORF:  2156 .. 2365  =  210 bp
ORF:  69 amino acids  =  8.1 kDa
ORF:  2546 .. 2803  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  2813 .. 3091  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  5912 .. 6064  =  153 bp
ORF:  50 amino acids  =  5.8 kDa
ORF:  6212 .. 6361  =  150 bp
ORF:  49 amino acids  =  5.8 kDa
ORF:  172 .. 846  =  675 bp
ORF:  224 amino acids  =  24.2 kDa
ORF:  1144 .. 1302  =  159 bp
ORF:  52 amino acids  =  5.8 kDa
ORF:  2059 .. 2256  =  198 bp
ORF:  65 amino acids  =  7.3 kDa
ORF:  3499 .. 3780  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2018 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter