Resources
Plasmid Files

MLM3639

Plasmid for expressing 3xFLAG®-tagged S. pyogenes Cas9 from either the CMV promoter in vivo or the T7 promoter in vitro.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

MLM3639 Sequence and MapMLM3639.dna
Map and Sequence File   
Sequence Author:  Joung Lab / Addgene #42252
Download Free Trial Get SnapGene Viewer

 PmeI (4653) SV40 NLS MLM3639 7614 bp
PmeI  (4653)
1 site
G T T T A A A C C A A A T T T G
Cas9
409 .. 4512  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
409 .. 4512  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6131 .. 6922  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6923 .. 6991  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
AmpR promoter
6992 .. 7096  =  105 bp
AmpR promoter
6992 .. 7096  =  105 bp
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter