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Plasmid Files

VP12

Mammalian cell plasmid for expressing the high-fidelity S. pyogenes Cas9 variant SpCas9-HF1.

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VP12.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Joung Lab / Addgene #72247
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SpeI (7380) MluI (7359) SgrDI (7125) ScaI (6684) PvuI (6574) BsaI (6265) AhdI (6204) BspQI - SapI (5195) BbsI (4864) PmeI (4653) AgeI (4623) PstI (4617) PsiI (4571) PaeR7I - XhoI (4539) BamHI (4513) BmtI (4055) NheI (4051) EagI - NotI - SacII (370) BstZ17I (623) BmgBI (628) MscI (679) Bsu36I (859) BstEII (1123) StuI (1148) BsiWI (1217) KasI (1350) NarI (1351) SfoI (1352) PluTI (1354) BfuAI - BspMI (1477) BsrGI (1967) PfoI * (2225) EcoRV (2250) FspAI (3359) BsgI (3727) VP12 7614 bp