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Plasmid Files

hCas9

Mammalian vector with a G418 resistance marker for expressing human codon-optimized Cas9.

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hCas9.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Church Lab / Addgene #41815
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MfeI - MunI (9529) SgrDI (9366) AhdI - AspEI - BmeRI - DriI - Eam1105I (8445) BssNAI - Bst1107I - BstZ17I (7173) CpoI - CspI - RsrII - Rsr2I (6715) AspA2I - AvrII - BlnI - XmaJI (5989) AatI - Eco147I - PceI - SseBI - StuI (5988) CsiI - MabI - SexAI * (5756) AdeI - DraIII (5466) MssI - PmeI (4921) AgeI - AsiGI - BshTI - CspAI - PinAI (4903) MluI (43) AhlI - BcuI - SpeI (64) BstSNI - Eco105I - SnaBI (405) XbaI (723) Eco32I - EcoRV (777) SbfI - SdaI - Sse8387I (995) XcmI (1099) BlpI - Bpu1102I - Bsp1720I - CelII (1696) AxyI - Bse21I - Bsu36I - Eco81I (2014) BamHI (2753) AjiI - BmgBI - BtrI (3258) AcvI - BbrPI - BstXI - Eco72I - PmaCI - PmlI - PspCI (3537) FspAI (3698) AarI (4008) PaeR7I - Sfr274I - SlaI - XhoI (4291) EcoRI (4570) AscI - PalAI - SgsI (4703) hCas9 9553 bp