lentiCas9-EGFP

Zhang lab lentiviral vector for expressing human codon-optimized S. pyogenes Cas9 plus enhanced GFP (EGFP).

Sequence Author: Sharp

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
BleoR EM7 promoter 5' LTR (truncated) FLAG CMV enhancer lentiCas9-EGFP 13,177 bp
Cas9
2886 .. 6989  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
2886 .. 6989  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
6990 .. 7037  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
6990 .. 7037  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
FLAG
7038 .. 7061  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
7038 .. 7061  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
P2A
7071 .. 7127  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
P2A
7071 .. 7127  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
EGFP
7128 .. 7844  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 1:  1a  
   7128 .. 7130  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
7128 .. 7844  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 2:  
   7131 .. 7844  =  714 bp
   237 amino acids  =  26.7 kDa
Product: enhanced GFP
mammalian codon-optimized
EGFP
7128 .. 7844  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
Product: enhanced GFP
mammalian codon-optimized
AmpR
12,184 .. 13,044  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   12,184 .. 12,975  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
12,184 .. 13,044  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   12,976 .. 13,044  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
12,184 .. 13,044  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
WPRE
7869 .. 8457  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
7869 .. 8457  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
11,425 .. 12,013  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
11,425 .. 12,013  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
9464 .. 9892  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
9464 .. 9892  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
238 .. 617  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
238 .. 617  =  380 bp
human cytomegalovirus immediate early enhancer
BleoR
10,349 .. 10,723  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
10,349 .. 10,723  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
9906 .. 10,235  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
9906 .. 10,235  =  330 bp
SV40 enhancer and early promoter
RRE
1680 .. 1913  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1680 .. 1913  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
bGH poly(A) signal
9194 .. 9418  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
9194 .. 9418  =  225 bp
bovine growth hormone polyadenylation signal
EF-1α core promoter
2650 .. 2861  =  212 bp
core promoter for human elongation factor EF-1α
EF-1α core promoter
2650 .. 2861  =  212 bp
core promoter for human elongation factor EF-1α
CMV promoter
619 .. 817  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
619 .. 817  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR (truncated)
835 .. 1015  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
835 .. 1015  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
8982 .. 9162  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
8982 .. 9162  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1062 .. 1187  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
1062 .. 1187  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
10,853 .. 10,974  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
10,853 .. 10,974  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
2440 .. 2557  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2440 .. 2557  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
13,045 .. 13,149  =  105 bp
AmpR promoter
13,045 .. 13,149  =  105 bp
EM7 promoter
10,283 .. 10,330  =  48 bp
synthetic bacterial promoter
EM7 promoter
10,283 .. 10,330  =  48 bp
synthetic bacterial promoter
SV40 ori
10,086 .. 10,221  =  136 bp
SV40 origin of replication
SV40 ori
10,086 .. 10,221  =  136 bp
SV40 origin of replication
ORF:  1558 .. 2445  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  7972 .. 8484  =  513 bp
ORF:  170 amino acids  =  18.0 kDa
ORF:  9415 .. 9681  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  10,349 .. 10,723  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  12,314 .. 12,580  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2886 .. 7844  =  4959 bp
ORF:  1652 amino acids  =  190.1 kDa
ORF:  7959 .. 9008  =  1050 bp
ORF:  349 amino acids  =  39.4 kDa
ORF:  4235 .. 4528  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  4799 .. 5248  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  10,342 .. 10,629  =  288 bp
ORF:  95 amino acids  =  10.1 kDa
ORF:  12,184 .. 13,044  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1473 .. 1853  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
ORF:  2598 .. 5405  =  2808 bp
ORF:  935 amino acids  =  98.0 kDa
Click here to try SnapGene

Download lentiCas9-EGFP.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.