pAAV-Guide-it-Down Vector (Linear)

CRISPR/Cas9 adeno-associated virus (AAV) vector for cloning and expression of a single guide RNA (sgRNA). Use together with pAAV-Guide-it-Up Vector.

Sequence Author: Clontech (TaKaRa)

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Guide-it™ Sequencing Primer 1 (6227 .. 6250) SV40 NLS polIII terminator pAAV-Guide-it-Down Vector 6306 bp
Guide-it™ Sequencing Primer 1
24-mer  /  38% GC
1 binding site
6227 .. 6250  =  24 annealed bases
Tm  =  56°C
Cas9-Down
2774 .. 5389  =  2616 bp
872 amino acids  =  101.2 kDa
Product: downstream fragment of Streptococcus pyogenes Cas9
Cas9-Down
2774 .. 5389  =  2616 bp
872 amino acids  =  101.2 kDa
Product: downstream fragment of Streptococcus pyogenes Cas9
SV40 NLS
5405 .. 5425  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5405 .. 5425  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
FLAG
5426 .. 5449  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
5426 .. 5449  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
AmpR
660 .. 1520  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   660 .. 728  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
660 .. 1520  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   729 .. 1520  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
660 .. 1520  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1691 .. 2279  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1691 .. 2279  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGH poly(A) signal
5461 .. 5937  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5461 .. 5937  =  477 bp
human growth hormone polyadenylation signal
U6 promoter
6060 .. 6300  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
6060 .. 6300  =  241 bp
RNA polymerase III promoter for human U6 snRNA
AAV2 ITR
153 .. 293  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
153 .. 293  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2624 .. 2764  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2624 .. 2764  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
555 .. 659  =  105 bp
AmpR promoter
555 .. 659  =  105 bp
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
ORF:  167 .. 448  =  282 bp
ORF:  93 amino acids  =  9.9 kDa
ORF:  2885 .. 5452  =  2568 bp
ORF:  855 amino acids  =  99.0 kDa
ORF:  660 .. 1520  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1124 .. 1390  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3250 .. 3648  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
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Download pAAV-Guide-it-Down Vector (Linear).dna file

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