pAC149-pCR8-dCas9VP160

Gateway® entry vector with a cassette encoding catalytically inactive dCas9 fused to the VP160 transcriptional activation domain.

Sequence Author: Jaenisch Lab / Addgene #48221

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rrnB T2 terminator HA pAC149-pCR8-dCas9VP160 7442 bp
ATG
689 .. 691  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
689 .. 691  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
HA
692 .. 718  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
692 .. 718  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
722 .. 742  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
722 .. 742  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
752 .. 4852  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 1:  
   752 .. 775  =  24 bp
   8 amino acids  =  923.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
752 .. 4852  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 2:  
   776 .. 778  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
752 .. 4852  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 3:  
   779 .. 3265  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
752 .. 4852  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 4:  
   3266 .. 3268  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
752 .. 4852  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 5:  
   3269 .. 4852  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
752 .. 4852  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
4856 .. 4876  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4856 .. 4876  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
VP160
4910 .. 5293  =  384 bp
128 amino acids  =  14.0 kDa
Product: 10 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)
VP160
4910 .. 5293  =  384 bp
128 amino acids  =  14.0 kDa
Product: 10 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)
SmR
5908 .. 6699  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
5908 .. 6699  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori
6792 .. 7380  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6792 .. 7380  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
attL2
5321 .. 5420  =  100 bp
recombination site for the Gateway® LR reaction
attL2
5321 .. 5420  =  100 bp
recombination site for the Gateway® LR reaction
attL1
569 .. 668  =  100 bp
recombination site for the Gateway® LR reaction
attL1
569 .. 668  =  100 bp
recombination site for the Gateway® LR reaction
rrnB T1 terminator
387 .. 473  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
387 .. 473  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T2 terminator
268 .. 295  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
268 .. 295  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
ORF:  628 .. 1080  =  453 bp
ORF:  150 amino acids  =  17.4 kDa
ORF:  5689 .. 6699  =  1011 bp
ORF:  336 amino acids  =  37.5 kDa
ORF:  641 .. 5305  =  4665 bp
ORF:  1554 amino acids  =  178.6 kDa
ORF:  5076 .. 5309  =  234 bp
ORF:  77 amino acids  =  9.0 kDa
ORF:  5940 .. 6197  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  6207 .. 6485  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  394 .. 690  =  297 bp
ORF:  98 amino acids  =  10.8 kDa
ORF:  2098 .. 2391  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  2662 .. 3111  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  4837 .. 5250  =  414 bp
ORF:  137 amino acids  =  14.2 kDa
ORF:  5947 .. 6330  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
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Download pAC149-pCR8-dCas9VP160.dna file

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