pACT2-CAS9c

High-copy yeast plasmid for expressing S. pyogenes Cas9 under control of the ADH1 promoter and terminator.

Sequence Author: Zhao Lab / Addgene #51055

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pACT2-CAS9c 11,495 bp
Cas9
2618 .. 6721  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
2618 .. 6721  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
6734 .. 6754  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6734 .. 6754  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
2μ ori
9916 .. 11,258  =  1343 bp
yeast 2μ plasmid origin of replication
2μ ori
9916 .. 11,258  =  1343 bp
yeast 2μ plasmid origin of replication
LEU2
7928 .. 9022  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
LEU2
7928 .. 9022  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
AmpR
131 .. 991  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   131 .. 199  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
131 .. 991  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   200 .. 991  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
131 .. 991  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1162 .. 1750  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1162 .. 1750  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
LEU2 promoter
9035 .. 9439  =  405 bp
LEU2 promoter
9035 .. 9439  =  405 bp
ADH1 promoter
2218 .. 2614  =  397 bp
promoter for alcohol dehydrogenase 1
ADH1 promoter
2218 .. 2614  =  397 bp
promoter for alcohol dehydrogenase 1
ADH1 terminator
6890 .. 7077  =  188 bp
transcription terminator for alcohol dehydrogenase 1
ADH1 terminator
6890 .. 7077  =  188 bp
transcription terminator for alcohol dehydrogenase 1
AmpR promoter
26 .. 130  =  105 bp
AmpR promoter
26 .. 130  =  105 bp
ORF:  9364 .. 9765  =  402 bp
ORF:  133 amino acids  =  15.9 kDa
ORF:  131 .. 991  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  2618 .. 6757  =  4140 bp
ORF:  1379 amino acids  =  159.7 kDa
ORF:  6684 .. 6911  =  228 bp
ORF:  75 amino acids  =  8.6 kDa
ORF:  8049 .. 8360  =  312 bp
ORF:  103 amino acids  =  11.1 kDa
ORF:  9951 .. 10,241  =  291 bp
ORF:  96 amino acids  =  11.5 kDa
ORF:  11,079 .. 11,420  =  342 bp
ORF:  113 amino acids  =  13.0 kDa
ORF:  2609 .. 3097  =  489 bp
ORF:  162 amino acids  =  18.2 kDa
ORF:  4022 .. 4540  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  4604 .. 5137  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  6131 .. 6664  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  7928 .. 9034  =  1107 bp
ORF:  368 amino acids  =  39.4 kDa
ORF:  595 .. 861  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7033 .. 7293  =  261 bp
ORF:  86 amino acids  =  10.0 kDa
ORF:  9697 .. 9936  =  240 bp
ORF:  79 amino acids  =  9.8 kDa
ORF:  10,717 .. 11,037  =  321 bp
ORF:  106 amino acids  =  12.9 kDa
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