Plasmid Files

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BsaI  (1245) 2 sites

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

BsaI  (1704) 2 sites

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

iCas9 2215 .. 6315  =  4101 bp 1367 amino acids  =  158.4 kDa Product: improved Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system; includes the accidental D147Y and P411T mutations generates RNA-guided double strand breaks in DNA

iCas9 2215 .. 6315  =  4101 bp 1367 amino acids  =  158.4 kDa Product: improved Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system; includes the accidental D147Y and P411T mutations generates RNA-guided double strand breaks in DNA

2μ ori 9396 .. 10,738  =  1343 bp yeast 2μ plasmid origin of replication

2μ ori 9396 .. 10,738  =  1343 bp yeast 2μ plasmid origin of replication

AmpR 8404 .. 9264  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      8404 .. 9195  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 8404 .. 9264  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      9196 .. 9264  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 8404 .. 9264  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

URA3 54 .. 857  =  804 bp 267 amino acids  =  29.3 kDa Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)

URA3 54 .. 857  =  804 bp 267 amino acids  =  29.3 kDa Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)

ori 7645 .. 8233  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 7645 .. 8233  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

RPR1 promoter 6655 .. 7061  =  407 bp promoter for the S. cerevisiae RNase P RNA gene

RPR1 promoter 6655 .. 7061  =  407 bp promoter for the S. cerevisiae RNase P RNA gene

TEF1 promoter 1757 .. 2155  =  399 bp promoter for EF-1α

TEF1 promoter 1757 .. 2155  =  399 bp promoter for EF-1α

ADH2 terminator 6343 .. 6642  =  300 bp transcription terminator for the S. cerevisiae alcohol dehydrogenase 2 (ADH2) gene

ADH2 terminator 6343 .. 6642  =  300 bp transcription terminator for the S. cerevisiae alcohol dehydrogenase 2 (ADH2) gene

lacZα 1257 .. 1553  =  297 bp 98 amino acids  =  11.0 kDa Product: LacZα fragment of β-galactosidase

lacZα 1257 .. 1553  =  297 bp 98 amino acids  =  11.0 kDa Product: LacZα fragment of β-galactosidase

SNR52 promoter 946 .. 1214  =  269 bp promoter for the S. cerevisiae small nucleolar RNA gene SNR52

SNR52 promoter 946 .. 1214  =  269 bp promoter for the S. cerevisiae small nucleolar RNA gene SNR52

AmpR promoter 9265 .. 9369  =  105 bp

AmpR promoter 9265 .. 9369  =  105 bp

tracrRNA 7062 .. 7140  =  79 bp trans-activating CRISPR RNA for the Streptococcus pyogenes CRISPR/Cas9 system

tracrRNA 7062 .. 7140  =  79 bp trans-activating CRISPR RNA for the Streptococcus pyogenes CRISPR/Cas9 system

RPR1 terminator 7148 .. 7210  =  63 bp transcription terminator for the S. cerevisiae RNase P RNA gene

RPR1 terminator 7148 .. 7210  =  63 bp transcription terminator for the S. cerevisiae RNase P RNA gene

truncated URA3 promoter 8 .. 53  =  46 bp Truncating this promoter forces yeast cells to maintain the plasmid at very high copy number.

truncated URA3 promoter 8 .. 53  =  46 bp Truncating this promoter forces yeast cells to maintain the plasmid at very high copy number.

5' DR 1217 .. 1249  =  33 bp 5' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system

5' DR 1217 .. 1249  =  33 bp 5' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system

lac promoter 1597 .. 1627  =  31 bp    Segment 3:  -10      1597 .. 1603  =  7 bp promoter for the E. coli lac operon

lac promoter 1597 .. 1627  =  31 bp    Segment 2:      1604 .. 1621  =  18 bp promoter for the E. coli lac operon

lac promoter 1597 .. 1627  =  31 bp    Segment 1:  -35      1622 .. 1627  =  6 bp promoter for the E. coli lac operon

lac promoter 1597 .. 1627  =  31 bp 3 segments promoter for the E. coli lac operon

3' DR 1705 .. 1735  =  31 bp 3' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system

3' DR 1705 .. 1735  =  31 bp 3' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system

FLAG 2167 .. 2190  =  24 bp 8 amino acids  =  1.0 kDa Product: FLAG® epitope tag, followed by an enterokinase cleavage site

FLAG 2167 .. 2190  =  24 bp 8 amino acids  =  1.0 kDa Product: FLAG® epitope tag, followed by an enterokinase cleavage site

SV40 NLS 2194 .. 2214  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 2194 .. 2214  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 6319 .. 6339  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 6319 .. 6339  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SUP4 terminator 1736 .. 1755  =  20 bp transcription terminator for the S. cerevisiae SUP4 tRNA gene

SUP4 terminator 1736 .. 1755  =  20 bp transcription terminator for the S. cerevisiae SUP4 tRNA gene

lac operator 1573 .. 1589  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 1573 .. 1589  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

ATG 2164 .. 2166  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 2164 .. 2166  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

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