Plasmid Files |
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![]() | pCRCT.dna |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
iCas9 2215 .. 6315 = 4101 bp 1367 amino acids = 158.4 kDa Product: improved Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system; includes the accidental D147Y and P411T mutations generates RNA-guided double strand breaks in DNA |
iCas9 2215 .. 6315 = 4101 bp 1367 amino acids = 158.4 kDa Product: improved Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system; includes the accidental D147Y and P411T mutations generates RNA-guided double strand breaks in DNA |
2μ ori 9396 .. 10,738 = 1343 bp yeast 2μ plasmid origin of replication |
2μ ori 9396 .. 10,738 = 1343 bp yeast 2μ plasmid origin of replication |
AmpR 8404 .. 9264 = 861 bp 286 amino acids = 31.6 kDa Segment 2: 8404 .. 9195 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 8404 .. 9264 = 861 bp 286 amino acids = 31.6 kDa Segment 1: signal sequence 9196 .. 9264 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 8404 .. 9264 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
URA3 54 .. 857 = 804 bp 267 amino acids = 29.3 kDa Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA) |
URA3 54 .. 857 = 804 bp 267 amino acids = 29.3 kDa Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA) |
ori 7645 .. 8233 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 7645 .. 8233 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
RPR1 promoter 6655 .. 7061 = 407 bp promoter for the S. cerevisiae RNase P RNA gene |
RPR1 promoter 6655 .. 7061 = 407 bp promoter for the S. cerevisiae RNase P RNA gene |
TEF1 promoter 1757 .. 2155 = 399 bp promoter for EF-1α |
TEF1 promoter 1757 .. 2155 = 399 bp promoter for EF-1α |
ADH2 terminator 6343 .. 6642 = 300 bp transcription terminator for the S. cerevisiae alcohol dehydrogenase 2 (ADH2) gene |
ADH2 terminator 6343 .. 6642 = 300 bp transcription terminator for the S. cerevisiae alcohol dehydrogenase 2 (ADH2) gene |
lacZα 1257 .. 1553 = 297 bp 98 amino acids = 11.0 kDa Product: LacZα fragment of β-galactosidase |
lacZα 1257 .. 1553 = 297 bp 98 amino acids = 11.0 kDa Product: LacZα fragment of β-galactosidase |
SNR52 promoter 946 .. 1214 = 269 bp promoter for the S. cerevisiae small nucleolar RNA gene SNR52 |
SNR52 promoter 946 .. 1214 = 269 bp promoter for the S. cerevisiae small nucleolar RNA gene SNR52 |
AmpR promoter 9265 .. 9369 = 105 bp |
AmpR promoter 9265 .. 9369 = 105 bp |
tracrRNA 7062 .. 7140 = 79 bp trans-activating CRISPR RNA for the Streptococcus pyogenes CRISPR/Cas9 system |
tracrRNA 7062 .. 7140 = 79 bp trans-activating CRISPR RNA for the Streptococcus pyogenes CRISPR/Cas9 system |
RPR1 terminator 7148 .. 7210 = 63 bp transcription terminator for the S. cerevisiae RNase P RNA gene |
RPR1 terminator 7148 .. 7210 = 63 bp transcription terminator for the S. cerevisiae RNase P RNA gene |
truncated URA3 promoter 8 .. 53 = 46 bp Truncating this promoter forces yeast cells to maintain the plasmid at very high copy number. |
truncated URA3 promoter 8 .. 53 = 46 bp Truncating this promoter forces yeast cells to maintain the plasmid at very high copy number. |
5' DR 1217 .. 1249 = 33 bp 5' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system |
5' DR 1217 .. 1249 = 33 bp 5' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system |
lac promoter 1597 .. 1627 = 31 bp Segment 3: -10 1597 .. 1603 = 7 bp promoter for the E. coli lac operon |
lac promoter 1597 .. 1627 = 31 bp Segment 2: 1604 .. 1621 = 18 bp promoter for the E. coli lac operon |
lac promoter 1597 .. 1627 = 31 bp Segment 1: -35 1622 .. 1627 = 6 bp promoter for the E. coli lac operon |
lac promoter 1597 .. 1627 = 31 bp 3 segments promoter for the E. coli lac operon |
3' DR 1705 .. 1735 = 31 bp 3' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system |
3' DR 1705 .. 1735 = 31 bp 3' portion of the direct repeat for the Streptococcus pyogenes CRISPR/Cas system |
FLAG 2167 .. 2190 = 24 bp 8 amino acids = 1.0 kDa Product: FLAG® epitope tag, followed by an enterokinase cleavage site |
FLAG 2167 .. 2190 = 24 bp 8 amino acids = 1.0 kDa Product: FLAG® epitope tag, followed by an enterokinase cleavage site |
SV40 NLS 2194 .. 2214 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 2194 .. 2214 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 6319 .. 6339 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 6319 .. 6339 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SUP4 terminator 1736 .. 1755 = 20 bp transcription terminator for the S. cerevisiae SUP4 tRNA gene |
SUP4 terminator 1736 .. 1755 = 20 bp transcription terminator for the S. cerevisiae SUP4 tRNA gene |
lac operator 1573 .. 1589 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 1573 .. 1589 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
ATG 2164 .. 2166 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 2164 .. 2166 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ORF: 2164 .. 6342 = 4179 bp ORF: 1392 amino acids = 161.4 kDa |
ORF: 8534 .. 8800 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 9431 .. 9721 = 291 bp ORF: 96 amino acids = 11.5 kDa |
ORF: 54 .. 857 = 804 bp ORF: 267 amino acids = 29.3 kDa |
ORF: 1257 .. 1553 = 297 bp ORF: 98 amino acids = 11.0 kDa |
ORF: 10,197 .. 10,517 = 321 bp ORF: 106 amino acids = 12.9 kDa |
ORF: 89 .. 565 = 477 bp ORF: 158 amino acids = 16.8 kDa |
ORF: 8404 .. 9264 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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