Resources
Plasmid Files

pCRISPR

Plasmid for reconstituting the S. pyogenes CRISPR/Cas system in E. coli. Use together with pCas9.

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pCRISPR Sequence and MappCRISPR.dna
Map and Sequence File   
Sequence Author:  Marraffini Lab / Addgene #42875
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 BsaI (2560) BsaI (2525) BsaI (2286) BsaI (2251) pCRISPR 2707 bp
BsaI  (2560)
4 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (2525)
4 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (2286)
4 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (2251)
4 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
NeoR/KanR
212 .. 1006  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
212 .. 1006  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
ori
1219 .. 1807  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1219 .. 1807  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
crRNA leader
2088 .. 2219  =  132 bp
crRNA leader sequence for the Streptococcus
pyogenes
CRISPR/Cas system
crRNA leader
2088 .. 2219  =  132 bp
crRNA leader sequence for the Streptococcus
pyogenes
CRISPR/Cas system
crRNA leader
2362 .. 2493  =  132 bp
crRNA leader sequence for the Streptococcus
pyogenes
CRISPR/Cas system
crRNA leader
2362 .. 2493  =  132 bp
crRNA leader sequence for the Streptococcus
pyogenes
CRISPR/Cas system
lambda t0 terminator
1037 .. 1131  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
1037 .. 1131  =  95 bp
transcription terminator from phage lambda
DR
2220 .. 2255  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2220 .. 2255  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2286 .. 2321  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2286 .. 2321  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2494 .. 2529  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2494 .. 2529  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2560 .. 2595  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
2560 .. 2595  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
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