pCS2-Cas9

S. pyogenes Cas9 plasmid for in vivo expression or in vitro mRNA production.

Sequence Author: Schier Lab / Addgene #47322

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pCS2-Cas9 8215 bp
SV40 NLS
540 .. 560  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
540 .. 560  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
573 .. 4676  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
573 .. 4676  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
CMV IE94 promoter
4774 .. 5751  =  978 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
CMV IE94 promoter
4774 .. 5751  =  978 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
AmpR
6415 .. 7275  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6415 .. 6483  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6415 .. 7275  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6484 .. 7275  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6415 .. 7275  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7446 .. 8034  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7446 .. 8034  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5829 .. 6284  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5829 .. 6284  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 poly(A) signal
373 .. 507  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
373 .. 507  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6310 .. 6414  =  105 bp
AmpR promoter
6310 .. 6414  =  105 bp
SP6 promoter
4721 .. 4739  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
4721 .. 4739  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  6415 .. 7275  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  630 .. 1163  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  2157 .. 2690  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  2754 .. 3272  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  4197 .. 4727  =  531 bp
ORF:  176 amino acids  =  19.5 kDa
ORF:  4839 .. 5120  =  282 bp
ORF:  93 amino acids  =  10.1 kDa
ORF:  5463 .. 5816  =  354 bp
ORF:  117 amino acids  =  12.9 kDa
ORF:  4820 .. 5092  =  273 bp
ORF:  90 amino acids  =  9.5 kDa
ORF:  5492 .. 5740  =  249 bp
ORF:  82 amino acids  =  9.1 kDa
ORF:  537 .. 4676  =  4140 bp
ORF:  1379 amino acids  =  159.7 kDa
ORF:  5325 .. 5849  =  525 bp
ORF:  174 amino acids  =  19.1 kDa
ORF:  6879 .. 7145  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pCS2-Cas9.dna file

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