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Plasmid Files

pCS2-nCas9n

Plasmid for expressing zebrafish codon-optimized S. pyogenes Cas9 with N- and C-terminal nuclear localization signals.

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pCS2-nCas9n Sequence and MappCS2-nCas9n.dna
Map and Sequence File   
Sequence Author:  Chen Lab / Addgene #47929
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 NotI (4523) ATG pCS2-nCas9n 8272 bp
NotI  (4523)
1 site
G C G G C C G C C G C C G G C G
Cas9
144 .. 4244  =  4101 bp
1367 amino acids  =  158.2 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
144 .. 4244  =  4101 bp
1367 amino acids  =  158.2 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
CMV IE94 promoter
7288 .. 8272  =  985 bp
enhancer/promoter region of simian
cytomegalovirus major immediate early
transcription unit IE94
CMV IE94 promoter
7288 .. 8272  =  985 bp
enhancer/promoter region of simian
cytomegalovirus major immediate early
transcription unit IE94
AmpR
5768 .. 6628  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   5768 .. 6559  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5768 .. 6628  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6560 .. 6628  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5768 .. 6628  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
5009 .. 5597  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5009 .. 5597  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
6759 .. 7214  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
6759 .. 7214  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 poly(A) signal
4321 .. 4455  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4321 .. 4455  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6629 .. 6733  =  105 bp
AmpR promoter
6629 .. 6733  =  105 bp
SV40 NLS
117 .. 137  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
117 .. 137  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
4260 .. 4280  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
4260 .. 4280  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SP6 promoter
35 .. 53  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
35 .. 53  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ATG
105 .. 107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
105 .. 107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
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