Resources
Plasmid Files

pCas9

Plasmid for reconstituting the S. pyogenes CRISPR/Cas system in E. coli. Use together with pCRISPR.

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pCas9 Sequence and MappCas9.dna
Map and Sequence File   
Sequence Author:  Marraffini Lab / Addgene #42876
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 DR BsaI (6550) BsaI (6515) DR pCas9 9326 bp
BsaI  (6550)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (6515)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
Cas9
2225 .. 6331  =  4107 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
2225 .. 6331  =  4107 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
CmR
8886 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
8886 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
p15A ori
848 .. 1393  =  546 bp
Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
p15A ori
848 .. 1393  =  546 bp
Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
crRNA leader
6352 .. 6483  =  132 bp
crRNA leader sequence for the Streptococcus
pyogenes
CRISPR/Cas system
crRNA leader
6352 .. 6483  =  132 bp
crRNA leader sequence for the Streptococcus
pyogenes
CRISPR/Cas system
cat promoter
220 .. 322  =  103 bp
promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase
cat promoter
220 .. 322  =  103 bp
promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase
tracrRNA
1851 .. 1929  =  79 bp
trans-activating CRISPR RNA for the Streptococcus
pyogenes
CRISPR/Cas9 system
tracrRNA
1851 .. 1929  =  79 bp
trans-activating CRISPR RNA for the Streptococcus
pyogenes
CRISPR/Cas9 system
DR
6484 .. 6519  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
6484 .. 6519  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
6550 .. 6585  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
DR
6550 .. 6585  =  36 bp
direct repeat for the Streptococcus pyogenes
CRISPR/Cas system
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