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Plasmid Files

pDCC6

Drosophila CRISPR/Cas9 genome editing vector for expressing a single guide RNA (sgRNA) together with Cas9.

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pDCC6 Sequence and MappDCC6.dna
Map and Sequence File   
Sequence Author:  Duchek Lab / Addgene #59985
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 BbsI (479) BbsI (501) polIII terminator ATG SV40 NLS pDCC6 8154 bp
BbsI  (479)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (501)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Cas9
1225 .. 5325  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes MGAS15252 Type II
CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1225 .. 5325  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes MGAS15252 Type II
CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
AmpR
6353 .. 7213  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6353 .. 6421  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6353 .. 7213  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6422 .. 7213  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6353 .. 7213  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
7384 .. 7972  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
7384 .. 7972  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
dU6 promoter
84 .. 483  =  400 bp
RNA polymerase III promoter for Drosophila U6-2
snRNA (Das et al., 1987)
dU6 promoter
84 .. 483  =  400 bp
RNA polymerase III promoter for Drosophila U6-2
snRNA (Das et al., 1987)
hsp70 promoter
663 .. 1004  =  342 bp
Drosophila melanogaster hsp70Bb promoter
hsp70 promoter
663 .. 1004  =  342 bp
Drosophila melanogaster hsp70Bb promoter
SV40 poly(A) signal
6122 .. 6256  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
6122 .. 6256  =  135 bp
SV40 polyadenylation signal
gRNA scaffold
502 .. 577  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
502 .. 577  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
3xFLAG
1108 .. 1173  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
3xFLAG
1108 .. 1173  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
SV40 NLS
1180 .. 1200  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
1180 .. 1200  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
5329 .. 5349  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
5329 .. 5349  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
polIII terminator
578 .. 583  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
578 .. 583  =  6 bp
RNA polymerase III transcription terminator
ATG
1105 .. 1107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1105 .. 1107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
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