pHAGE EF1-alpha dCas9-VP64

Lentiviral CRISPR activation vector for constitutive expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain.

Sequence Author: Maehr

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
attB2 pHAGE EF1 α dCas9-VP64 12,794 bp
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   8340 .. 8366  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   8367 .. 8369  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   8370 .. 10,856  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   10,857 .. 10,859  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   10,860 .. 12,443  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
12,456 .. 12,476  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
12,456 .. 12,476  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
3xHA
12,477 .. 12,566  =  90 bp
30 amino acids  =  3.5 kDa
Product: three tandem HA epitope tags
3xHA
12,477 .. 12,566  =  90 bp
30 amino acids  =  3.5 kDa
Product: three tandem HA epitope tags
VP64
12,588 .. 12,737  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
VP64
12,588 .. 12,737  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
EF-1α promoter
7093 .. 8271  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
7093 .. 8271  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
2468 .. 3328  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   2468 .. 2536  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2468 .. 3328  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   2537 .. 3328  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2468 .. 3328  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
4873 .. 5506  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
4873 .. 5506  =  634 bp
long terminal repeat (LTR) from HIV-1
PuroR
646 .. 1245  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
646 .. 1245  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
1262 .. 1850  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
1262 .. 1850  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
3499 .. 4087  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3499 .. 4087  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
126 .. 625  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
126 .. 625  =  500 bp
mouse phosphoglycerate kinase 1 promoter
LTR (ΔU3)
1925 .. 2158  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
1925 .. 2158  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
RRE
6175 .. 6408  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
6175 .. 6408  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
HIV-1 Ψ
5553 .. 5678  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5553 .. 5678  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
6930 .. 7047  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
6930 .. 7047  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
2363 .. 2467  =  105 bp
AmpR promoter
2363 .. 2467  =  105 bp
attB1
8306 .. 8330  =  25 bp
recombination site for the Gateway® BP reaction
attB1
8306 .. 8330  =  25 bp
recombination site for the Gateway® BP reaction
attB2
12,784 .. 14  =  25 bp
recombination site for the Gateway® BP reaction
attB2
12,784 .. 14  =  25 bp
recombination site for the Gateway® BP reaction
EF-1α intron A
7324 .. 8262  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
7324 .. 8262  =  939 bp
intron upstream of the start codon of human EF-1α
ORF:  406 .. 1245  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  6910 .. 7170  =  261 bp
ORF:  86 amino acids  =  9.7 kDa
ORF:  12,406 .. 12,738  =  333 bp
ORF:  110 amino acids  =  12.5 kDa
ORF:  1352 .. 1864  =  513 bp
ORF:  170 amino acids  =  18.6 kDa
ORF:  2468 .. 3328  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  6053 .. 6979  =  927 bp
ORF:  308 amino acids  =  35.4 kDa
ORF:  7649 .. 8086  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  1365 .. 1898  =  534 bp
ORF:  177 amino acids  =  18.8 kDa
ORF:  7728 .. 7961  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  8340 .. 12,746  =  4407 bp
ORF:  1468 amino acids  =  169.2 kDa
ORF:  6753 .. 7001  =  249 bp
ORF:  82 amino acids  =  9.0 kDa
ORF:  8259 .. 8750  =  492 bp
ORF:  163 amino acids  =  17.9 kDa
ORF:  9744 .. 10,262  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  10,326 .. 10,601  =  276 bp
ORF:  91 amino acids  =  10.3 kDa
ORF:  11,229 .. 12,386  =  1158 bp
ORF:  385 amino acids  =  43.7 kDa
ORF:  7391 .. 8011  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  2932 .. 3198  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5887 .. 6348  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
Click here to try SnapGene

Download pHAGE EF1-alpha dCas9-VP64.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.