pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 24 copies of an optimized GCN4_v4 peptide.

Sequence Author: Vale Lab / Addgene #60910

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SV40 NLS SV40 NLS SV40 NLS SV40 NLS ATG pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 14,911 bp
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   7810 .. 7836  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   7837 .. 7839  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   7840 .. 10,326  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   10,327 .. 10,329  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   10,330 .. 11,913  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 1:  GCN4_v4  
   12,088 .. 12,144  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 2:  
   12,145 .. 12,159  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 3:  GCN4_v4  
   12,160 .. 12,216  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 4:  
   12,217 .. 12,231  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 5:  GCN4_v4  
   12,232 .. 12,288  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 6:  
   12,289 .. 12,303  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 7:  GCN4_v4  
   12,304 .. 12,360  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 8:  
   12,361 .. 12,375  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 9:  GCN4_v4  
   12,376 .. 12,432  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 10:  
   12,433 .. 12,447  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 11:  GCN4_v4  
   12,448 .. 12,504  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 12:  
   12,505 .. 12,519  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 13:  GCN4_v4  
   12,520 .. 12,576  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 14:  
   12,577 .. 12,591  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 15:  GCN4_v4  
   12,592 .. 12,648  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 16:  
   12,649 .. 12,663  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 17:  GCN4_v4  
   12,664 .. 12,720  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 18:  
   12,721 .. 12,735  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 19:  GCN4_v4  
   12,736 .. 12,792  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 20:  
   12,793 .. 12,807  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 21:  GCN4_v4  
   12,808 .. 12,864  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 22:  
   12,865 .. 12,879  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 23:  GCN4_v4  
   12,880 .. 12,936  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 24:  
   12,937 .. 12,951  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 25:  GCN4_v4  
   12,952 .. 13,008  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 26:  
   13,009 .. 13,023  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 27:  GCN4_v4  
   13,024 .. 13,080  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 28:  
   13,081 .. 13,095  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 29:  GCN4_v4  
   13,096 .. 13,152  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 30:  
   13,153 .. 13,167  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 31:  GCN4_v4  
   13,168 .. 13,224  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 32:  
   13,225 .. 13,239  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 33:  GCN4_v4  
   13,240 .. 13,296  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 34:  
   13,297 .. 13,311  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 35:  GCN4_v4  
   13,312 .. 13,368  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 36:  
   13,369 .. 13,383  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 37:  GCN4_v4  
   13,384 .. 13,440  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 38:  
   13,441 .. 13,455  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 39:  GCN4_v4  
   13,456 .. 13,512  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 40:  
   13,513 .. 13,527  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 41:  GCN4_v4  
   13,528 .. 13,584  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 42:  
   13,585 .. 13,599  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 43:  GCN4_v4  
   13,600 .. 13,656  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 44:  
   13,657 .. 13,671  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 45:  GCN4_v4  
   13,672 .. 13,728  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 46:  
   13,729 .. 13,743  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 47:  GCN4_v4  
   13,744 .. 13,800  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
SV40 NLS
13,840 .. 13,860  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
13,840 .. 13,860  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
P2A
13,870 .. 13,926  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
P2A
13,870 .. 13,926  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
TagBFP
13,939 .. 14,640  =  702 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
TagBFP
13,939 .. 14,640  =  702 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1014 .. 1082  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1083 .. 1874  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2879 .. 3208  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2879 .. 3208  =  330 bp
SV40 enhancer and early promoter
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
LTR (ΔU3)
14,755 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
14,755 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
909 .. 1013  =  105 bp
AmpR promoter
909 .. 1013  =  105 bp
SV40 ori
3059 .. 3194  =  136 bp
SV40 origin of replication
SV40 ori
3059 .. 3194  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
ORF:  310 .. 672  =  363 bp
ORF:  120 amino acids  =  12.4 kDa
ORF:  4486 .. 4959  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  6460 .. 7557  =  1098 bp
ORF:  365 amino acids  =  42.1 kDa
ORF:  7732 .. 14,640  =  6909 bp
ORF:  2302 amino acids  =  260.6 kDa
ORF:  3491 .. 3865  =  375 bp
ORF:  124 amino acids  =  14.0 kDa
ORF:  1014 .. 1874  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3645 .. 3926  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
ORF:  3957 .. 4340  =  384 bp
ORF:  127 amino acids  =  14.9 kDa
ORF:  1478 .. 1744  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3539 .. 3958  =  420 bp
ORF:  139 amino acids  =  15.9 kDa
ORF:  226 .. 537  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  7774 .. 12,114  =  4341 bp
ORF:  1446 amino acids  =  152.5 kDa
ORF:  13,720 .. 14,445  =  726 bp
ORF:  241 amino acids  =  25.5 kDa
ORF:  6441 .. 6755  =  315 bp
ORF:  104 amino acids  =  11.0 kDa
ORF:  7713 .. 7937  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  9159 .. 9452  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  9723 .. 10,172  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
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Download pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE.dna file

SnapGene

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Individual Sequences & Maps

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