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Plasmid Files

pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 24 copies of an optimized GCN4_v4 peptide.

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Vale Lab / Addgene #60910
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SbfI - SdaI - Sse8387I (14,668) SalI (14,658) PacI (14,637) AxyI - Bse21I - Bsu36I - Eco81I (14,192) CpoI - CspI - RsrII - Rsr2I (12,052) BamHI (12,043) SmaI (7797) Cfr9I - TspMI - XmaI - XmaCI (7795) Cfr42I - KspI - SacII - Sfr303I - SgrBI - SstII (7791) MluI (7758) EcoRI (7501) AsuNHI - NheI (340) BmtI - BspOI (344) SgrAI (382) AfeI - Aor51HI - Eco47III (468) BpvUI - Ple19I - PvuI (1432) Acc16I - AviII - FspI - NsbI (1578) SfiI (3146) AgeI - AsiGI - BshTI - CspAI - PinAI (3683) BstHPI - HpaI - KspAI (4981) BbvCI (6703) AleI - OliI (6856)